Vazquez-Guillen Jose Manuel, Palacios-Saucedo Gerardo C, Rivera-Morales Lydia Guadalupe, Caballero-Trejo Amilcar, Flores-Flores Aldo Sebastian, Quiroga-Garza Juan Manuel, Chavez-Santoscoy Rocio Alejandra, Hernandez-Perez Jesus, Hinojosa-Alvarez Silvia Alejandra, Hernandez-Gonzalez Julio Antonio, Rojas-Contreras Maurilia, Vazquez-Juarez Ricardo, Valladares-Trujillo Ramon, Alonso-Tellez Cesar Alejandro, Treviño-Baez Joaquin Dario, Rivera-Alvarado Miguel Angel, Tamez-Guerra Reyes S, Rodriguez-Padilla Cristina
Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, Laboratorio de Inmunologia y Virologia, San Nicolas de los Garza, Nuevo Leon, Mexico.
Division de Investigacion en Salud y Division de Auxiliares de Diagnostico, Unidad Medica de Alta Especialidad (UMAE) No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon, Mexico.
PeerJ. 2025 May 22;13:e19454. doi: 10.7717/peerj.19454. eCollection 2025.
(Group B , GBS) is an important pathogen associated with neonatal sepsis, pneumonia, and meningitis, which can be transmitted from colonized pregnant women to their newborns. This study aimed to determine the prevalence and characterize the genomic features of isolates from pregnant women attending a referral hospital in Northeastern Mexico.
Vaginal-rectal swabs were collected from pregnant women during routine prenatal care between April 2017 and March 2020. Whole-genome sequencing was conducted to determine sequence type (ST), clonal complex (CC), capsular polysaccharide (Cps) genotype, virulence factors, and antibiotic resistance genes through comparative genome analysis.
colonization was detected in 51 (2.7%) of 1,924 pregnant women. The most common STs were ST8 (23.5%) and ST88 (15.7%). Cps genotyping showed high concordance between serological and molecular methods. Genes conferring resistance to tetracyclines (, 60.1%) and macrolides (, 100%) were identified. Key virulence factor genes, including , , and , were present in over 90% of the isolates.
Although GBS colonization prevalence was low, genomic analysis revealed the genetic diversity of in Northeastern Mexico, emphasizing the importance of molecular techniques for epidemiological surveillance and infection control.
B族链球菌（GBS）是一种与新生儿败血症、肺炎和脑膜炎相关的重要病原体，可从定植的孕妇传播给其新生儿。本研究旨在确定墨西哥东北部一家转诊医院就诊的孕妇中GBS的流行率，并对其分离株的基因组特征进行表征。
在2017年4月至2020年3月的常规产前检查期间，从孕妇中采集阴道-直肠拭子。通过比较基因组分析进行全基因组测序，以确定序列类型（ST）、克隆复合体（CC）、荚膜多糖（Cps）基因型、毒力因子和抗生素耐药基因。
在1924名孕妇中，有51名（2.7%）检测到GBS定植。最常见的STs是ST8（23.5%）和ST88（15.7%）。Cps基因分型显示血清学和分子方法之间具有高度一致性。鉴定出对四环素（60.1%）和大环内酯类（100%）耐药的基因。超过90%的分离株中存在关键毒力因子基因，包括、和。
尽管GBS定植率较低，但基因组分析揭示了墨西哥东北部GBS的遗传多样性，强调了分子技术在流行病学监测和感染控制中的重要性。
