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使用培养和分子检测方法评估西班牙西北地区土壤样本中自由生活阿米巴的存在情况。

Assessment of the Presence of Free-Living Amoebae in Soil Samples from the Northwest Region of Spain Using Culture and Molecular Assays.

作者信息

Pérez-Pérez Patricia, Rodríguez-Escolar Iván, Córdoba-Lanús Elizabeth, Domínguez-de-Barros Angélica, García-Pérez Omar, Piñero José E, Morchón Rodrigo, Lorenzo-Morales Jacob

机构信息

Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias (IUETSPC), Universidad de La Laguna (ULL), 38206 San Cristóbal de La Laguna, Spain.

Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, 38200 San Cristóbal de La Laguna, Spain.

出版信息

Microorganisms. 2025 May 2;13(5):1065. doi: 10.3390/microorganisms13051065.

DOI:10.3390/microorganisms13051065
PMID:40431237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12114036/
Abstract

Free-living amoebae (FLA) such as spp., , , spp., spp., and are naturally widespread in the environment, causing rare but fatal and debilitating infections in humans. In the present study, a total of 87 soil samples were collected from four provinces in the autonomous community of Castilla y León, Spain. These samples were collected in three different seasons during 2022-2023 (t1-t3) and were analysed by culture and molecular techniques (conventional PCR/sanger sequencing and qPCR). The obtained data revealed that the genus and the species were the most prevalent FLA. Furthermore, other genera/species of FLA were identified in the tested soil sources, suggesting a rich microbial biodiversity in Castilla y León soils. In addition, this study provides an important basis for future research on the ecology of these organisms and their potential impact on public health and the environment.

摘要

自由生活阿米巴（FLA），如 属、 属、 属、 属、 属和 属，在环境中自然广泛存在，可导致人类罕见但致命且使人衰弱的感染。在本研究中，共从西班牙卡斯蒂利亚-莱昂自治区的四个省份采集了87份土壤样本。这些样本于2022 - 2023年的三个不同季节（t1 - t3）采集，并通过培养和分子技术（常规PCR/桑格测序和qPCR）进行分析。获得的数据显示， 属和 种是最普遍的FLA。此外，在测试的土壤样本中还鉴定出了其他FLA属/种，这表明卡斯蒂利亚-莱昂土壤中存在丰富的微生物生物多样性。此外，本研究为这些生物的生态学及其对公共卫生和环境的潜在影响的未来研究提供了重要依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/0c5dca114843/microorganisms-13-01065-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/fd77f8a50fd5/microorganisms-13-01065-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/f0b0e3d4cd79/microorganisms-13-01065-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/5ca298770ddc/microorganisms-13-01065-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/96b3b979ece8/microorganisms-13-01065-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/dedbeb470987/microorganisms-13-01065-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/5cf58d0f992d/microorganisms-13-01065-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/0c5dca114843/microorganisms-13-01065-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/fd77f8a50fd5/microorganisms-13-01065-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/f0b0e3d4cd79/microorganisms-13-01065-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/5ca298770ddc/microorganisms-13-01065-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/96b3b979ece8/microorganisms-13-01065-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/dedbeb470987/microorganisms-13-01065-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/5cf58d0f992d/microorganisms-13-01065-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea6f/12114036/0c5dca114843/microorganisms-13-01065-g007.jpg

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Vermamoeba vermiformis as the etiological agent in a patient with suspected non-Acanthamoeba keratitis.蠕形艾美耳球虫可能是疑似非棘阿米巴角膜炎患者的病因。
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