Krishnan Anagha, Dahlin Lukas R, Guarnieri Michael T, Weissman Joseph C, Posewitz Matthew C
National Renewable Energy Laboratory, Biosciences Center, Golden, CO 80401, USA.
Colorado School of Mines, Department of Chemistry, Golden, CO 80401, USA.
Trends Biotechnol. 2025 Apr;43(4):759-772. doi: 10.1016/j.tibtech.2024.10.004. Epub 2024 Nov 8.
The Picochlorum genus is a distinctive eukaryotic green-algal clade that is the focus of several current biotechnological studies. It is capable of extremely rapid growth rates and has exceptional tolerances to high salinity, intense light, and elevated temperatures. Importantly, it has robust stability and high-biomass productivities in outdoor field trials in seawater. These features have propelled Picochlorum into the spotlight as a promising model for both fundamental and biotechnological research. Recently, several genetic tools, including genome editing, were developed for these algae, enabling insights into Picochlorum photophysiology and algal transformations for expanded capabilities. Here, we survey the Picochlorum genus, its genetic toolbox, recently characterized transformants, and discuss the commercial potential of Picochlorum as a salt-water photoautotrophic biocatalyst.
皮考氯藻属是一种独特的真核绿藻进化枝，是当前多项生物技术研究的重点。它具有极快的生长速度，对高盐度、强光和高温具有非凡的耐受性。重要的是，在海水室外田间试验中，它具有强大的稳定性和高生物量生产力。这些特性使皮考氯藻成为基础研究和生物技术研究的一个有前景的模型而备受关注。最近，为这些藻类开发了几种遗传工具，包括基因组编辑，这有助于深入了解皮考氯藻的光生理学以及实现藻类转化以扩展其能力。在这里，我们概述了皮考氯藻属、其遗传工具箱、最近表征的转化体，并讨论了皮考氯藻作为盐水光合自养生物催化剂的商业潜力。
