Wang Ganen, Gong Han, Zou Yang, Zhang Haijiao, Mao Xueying
Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Tianjin Haihe Dairy Co., Ltd., Tianjin 300300, China.
Nutrients. 2025 May 8;17(10):1610. doi: 10.3390/nu17101610.
: Overuse or misuse of antibiotics could cause adverse effects such as gut microbiota dysbiosis and intestinal barrier dysfunction. Probiotic intervention could effectively alleviate these symptoms. However, the precise efficacy of subsp. PB200 ( PB200) in mitigating antibiotic-induced intestinal injury remains unclear. The aim of this study was to systematically evaluate the effects of PB200 on intestinal barrier injury and gut microbiota dysbiosis in a murine model of antibiotic-induced intestinal injury. BALB/c mice were administered ceftriaxone sodium via oral gavage for seven consecutive days, followed by probiotic intervention daily via gastric gavage for 4 weeks. The results indicated that PB200 played a positive role in enhancing intestinal barrier function, as evidenced by the restored intestinal morphology, and elevated the expression of tight junctions including ZO-1, Claudin-4 and Occludin (2.76-fold, 4.39-fold, and 2.61-fold, respectively) compared to that in the Model group. PB200 normalized the levels of serum pro- and anti-inflammatory factors, including IL-1β, IL-6, TNF-α and IL-10, and elevated the diversity and richness of gut microbiota. PB200 significantly elevated the levels of propionic acid and butyric acid, with increases of 1.67-fold and 2.82-fold, respectively, compared to the Model group. Notably, PB200 reduced the abundance of and increased the abundance of , promoting the rebalance of gut microbiota. Taken together, these findings highlighted the significant potential of PB200 in alleviating intestinal barrier damage and restoring the balance of gut microbiota caused by an antibiotic.
抗生素的过度使用或滥用可能会导致诸如肠道微生物群失调和肠道屏障功能障碍等不良反应。益生菌干预可以有效缓解这些症状。然而，PB200亚种在减轻抗生素诱导的肠道损伤方面的确切功效仍不清楚。本研究的目的是系统评估PB200对抗生素诱导的肠道损伤小鼠模型中肠道屏障损伤和肠道微生物群失调的影响。将头孢曲松钠通过口服灌胃连续7天给予BALB/c小鼠，随后每天通过胃管饲法进行益生菌干预，持续4周。结果表明，PB200在增强肠道屏障功能方面发挥了积极作用，肠道形态得以恢复就是证明，并且与模型组相比，紧密连接蛋白ZO-1、Claudin-4和Occludin的表达分别提高了2.76倍、4.39倍和2.61倍。PB200使血清促炎和抗炎因子（包括IL-1β、IL-6、TNF-α和IL-10）的水平正常化，并提高了肠道微生物群的多样性和丰富度。PB200显著提高了丙酸和丁酸的水平，与模型组相比分别增加了1.67倍和2.82倍。值得注意的是，PB200降低了某菌属的丰度并增加了另一菌属的丰度，促进了肠道微生物群的重新平衡。综上所述，这些发现突出了PB200在减轻抗生素引起的肠道屏障损伤和恢复肠道微生物群平衡方面的巨大潜力。
