Gökmen Tülin Güven, Ütük Armağan Erdem, Tokgöz Esra Aslan, Uprak Nur Sima, Tekin Afra Sena, Erol Ufuk, Demir Pınar Ayvazoğlu, Sezer Osman, Günaydın Elçin
Department of Microbiology, Ceyhan Veterinary Faculty, Çukurova University, Adana 01330, Türkiye.
Department of Parasitology, Ceyhan Veterinary Faculty, Çukurova University, Adana 01330, Türkiye.
Vet Sci. 2025 May 15;12(5):481. doi: 10.3390/vetsci12050481.
Anaplasmosis is a disease in animals that leads to significant economic losses. In addition, the zoonotic potential of vector-borne species is increasing its importance, both around the world and in Türkiye, in particular. The aim of this study was to determine the prevalence and phylogenetic profile of species in goats from the districts of Adana province, Türkiye, via molecular methods. For this purpose, blood samples from 364 goats of various breeds, ages, and genders were collected, and the presence of 55 spp. was determined through the PCR method. was detected in 55 samples (15.1%) and in 11 samples (3%) by Nested-PCR. However, was not detected. The phylogenetic relationships of and were investigated by DNA sequencing of the and gene regions. Through sequence analysis in , we identified nine isolates as -like 1 and one isolate as -like 2. Forty-six of the isolates were 100% similar to each other. The other 2 isolates were also 100% similar to each other but had a single nucleotide difference from the other 46 isolates. Notably, the isolate identified as -like 2 was detected for the first time in goats in Türkiye.
无形体病是一种会给动物带来重大经济损失的疾病。此外,媒介传播物种的人畜共患病潜力在全球范围内,尤其是在土耳其,正使其重要性日益增加。本研究的目的是通过分子方法确定土耳其阿达纳省各地区山羊中该物种的流行情况和系统发育概况。为此,采集了364只不同品种、年龄和性别的山羊的血样,并通过PCR方法确定了55种该物种的存在情况。通过巢式PCR在55个样本(15.1%)中检测到了该物种,在11个样本(3%)中检测到了另一种。然而,未检测到第三种。通过对该物种的相关基因区域进行DNA测序,研究了其与另一种的系统发育关系。通过在相关软件中的序列分析,我们鉴定出9个分离株为某一种类似1型,1个分离株为某一种类似2型。在该物种的分离株中,46个彼此100%相似。另外2个分离株也彼此100%相似,但与其他46个分离株有一个单核苷酸差异。值得注意的是,鉴定为某一种类似2型的分离株首次在土耳其的山羊中被检测到。