• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

LC4 原发性样细胞系的分类——重现 CDK4 过表达的免疫逃逸型 HIV-HCV 诱导的肝癌

Classification of the LC4 Primarily-like Cell Line-Recapitulating a CDK4 Overexpressing Immune Evasive HIV-HCV-Induced HCC.

作者信息

Kah Janine, Staffeldt Lisa, Volz Tassilo, Schulze Kornelius, Heumann Asmus, Rövenstrunk Götz, Goebel Meike, Peine Sven, Dandri Maura, Lüth Stefan

机构信息

Department of Internal Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.

Faculty of Health Sciences Brandenburg, Brandenburg Medical School Theodor Fontane, 16816 Neuruppin, Germany.

出版信息

Viruses. 2025 Apr 30;17(5):653. doi: 10.3390/v17050653.

DOI:10.3390/v17050653
PMID:40431665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12115383/
Abstract

BACKGROUND

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality. HCC is characterized by high heterogeneity and, subsequently, adaptation by developing resistance to current treatments. Applying individualized models is crucial to understanding the potential of approved therapies. Therefore, we classify a primary-like cell line derived from the core region of an HCC with underlying HIV-HCV co-infection employing deep analysis on the pathway regulation level.

METHODS

We employed DEG analysis, followed by pathway analysis, to characterize the preservation level of the LC4 cells and the level of adoption. Next, we classify the new model for HCC research by employing healthy donor samples, commonly used HCC cell lines, and global RNAseq datasets.

RESULTS

LC4 cells reflect the characteristics of the parental cancer region, including immunosuppression and metabolic reprogramming, characterized by the downregulation of drug-metabolizing enzymes compared to healthy individuals, indicating a transition to alternate metabolic pathways. Moreover, we identified specific biomarkers equally regulated in the parental tissue, in global datasets of the same entities as well as in LC4 cells.

CONCLUSIONS

We classified LC4 cells as an individual immunosuppressive and highly progressive primary-like HCC cell line. LC4 cells are applicable as a model for preclinical drug testing, minimizing the lack of preclinical models in HCV-HIV-induced HCC research.

摘要

背景

肝细胞癌（HCC）是癌症相关死亡的主要原因之一。HCC具有高度异质性，随后会通过对当前治疗产生耐药性来实现适应。应用个体化模型对于理解获批疗法的潜力至关重要。因此，我们通过对通路调控水平进行深入分析，对源自合并HIV-HCV感染的HCC核心区域的类原代细胞系进行分类。

方法

我们采用差异基因表达（DEG）分析，随后进行通路分析，以表征LC4细胞的保留水平和采用水平。接下来，我们通过使用健康供体样本、常用的HCC细胞系和全球RNA测序数据集，对HCC研究的新模型进行分类。

结果

LC4细胞反映了亲本癌区域的特征，包括免疫抑制和代谢重编程，与健康个体相比，其特征在于药物代谢酶的下调，表明向替代代谢途径的转变。此外，我们在亲本组织、相同实体的全球数据集中以及LC4细胞中鉴定出了受到同等调控的特定生物标志物。

结论

我们将LC4细胞分类为一种个体免疫抑制且高度进展性的类原代HCC细胞系。LC4细胞可作为临床前药物测试的模型，最大限度地减少HCV-HIV诱导的HCC研究中临床前模型的缺乏。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/b8d447dd75cd/viruses-17-00653-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/eb2d30ccdcc7/viruses-17-00653-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/b438907d3111/viruses-17-00653-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/e78a9b64e13d/viruses-17-00653-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/3fd3540f9454/viruses-17-00653-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/caf3a7e50559/viruses-17-00653-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/80348bf0837b/viruses-17-00653-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/46c0169529de/viruses-17-00653-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/3a175a23fd0b/viruses-17-00653-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/b8d447dd75cd/viruses-17-00653-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/eb2d30ccdcc7/viruses-17-00653-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/b438907d3111/viruses-17-00653-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/e78a9b64e13d/viruses-17-00653-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/3fd3540f9454/viruses-17-00653-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/caf3a7e50559/viruses-17-00653-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/80348bf0837b/viruses-17-00653-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/46c0169529de/viruses-17-00653-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/3a175a23fd0b/viruses-17-00653-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c89a/12115383/b8d447dd75cd/viruses-17-00653-g009.jpg

相似文献

1
Classification of the LC4 Primarily-like Cell Line-Recapitulating a CDK4 Overexpressing Immune Evasive HIV-HCV-Induced HCC.LC4 原发性样细胞系的分类——重现 CDK4 过表达的免疫逃逸型 HIV-HCV 诱导的肝癌
Viruses. 2025 Apr 30;17(5):653. doi: 10.3390/v17050653.
2
NIH Consensus Statement on Management of Hepatitis C: 2002.美国国立卫生研究院关于丙型肝炎管理的共识声明：2002年。
NIH Consens State Sci Statements. 2002;19(3):1-46.
3
Hepatocellular carcinoma xenograft supports HCV replication: a mouse model for evaluating antivirals.肝细胞癌异种移植支持 HCV 复制：一种用于评估抗病毒药物的小鼠模型。
World J Gastroenterol. 2011 Jan 21;17(3):300-12. doi: 10.3748/wjg.v17.i3.300.
4
Pharmacological interventions for acute hepatitis C infection: an attempted network meta-analysis.急性丙型肝炎感染的药物干预：一项网状Meta分析尝试
Cochrane Database Syst Rev. 2017 Mar 13;3(3):CD011644. doi: 10.1002/14651858.CD011644.pub2.
5
Arsenic trioxide augments immunogenic cell death and induces cGAS-STING-IFN pathway activation in hepatocellular carcinoma.三氧化二砷增强肝癌的免疫原性细胞死亡并诱导 cGAS-STING-IFN 通路激活。
Cell Death Dis. 2024 Apr 29;15(4):300. doi: 10.1038/s41419-024-06685-8.
6
Direct-acting antivirals for chronic hepatitis C.用于慢性丙型肝炎的直接作用抗病毒药物。
Cochrane Database Syst Rev. 2017 Sep 18;9(9):CD012143. doi: 10.1002/14651858.CD012143.pub3.
7
Multi-omics analysis identifies SNP-associated immune-related signatures by integrating Mendelian randomization and machine learning in hepatocellular carcinoma.多组学分析通过整合孟德尔随机化和机器学习在肝细胞癌中识别与单核苷酸多态性相关的免疫相关特征。
Sci Rep. 2025 Jul 4;15(1):23930. doi: 10.1038/s41598-025-09010-1.
8
Genotype and Association of XRCC1 Gene With Liver Disease in Chronic Hcv Patients.慢性丙型肝炎患者XRCC1基因的基因型及其与肝脏疾病的关联
Asian Pac J Cancer Prev. 2025 Apr 1;26(4):1385-1391. doi: 10.31557/APJCP.2025.26.4.1385.
9
Surveillance of cirrhosis for hepatocellular carcinoma: systematic review and economic analysis.肝细胞癌肝硬化监测：系统评价与经济分析
Health Technol Assess. 2007 Sep;11(34):1-206. doi: 10.3310/hta11340.
10
Percutaneous ethanol injection or percutaneous acetic acid injection for early hepatocellular carcinoma.经皮乙醇注射或经皮乙酸注射治疗早期肝细胞癌。
Cochrane Database Syst Rev. 2015 Jan 26;1(1):CD006745. doi: 10.1002/14651858.CD006745.pub3.

本文引用的文献

1
Hepatocellular carcinoma recurrence: Predictors and management.肝细胞癌复发：预测因素与管理
Liver Res. 2023 Nov 19;7(4):321-332. doi: 10.1016/j.livres.2023.11.004. eCollection 2023 Dec.
2
Decoding the Role of CYP450 Enzymes in Metabolism and Disease: A Comprehensive Review.解读细胞色素P450酶在代谢与疾病中的作用：全面综述
Biomedicines. 2024 Jul 2;12(7):1467. doi: 10.3390/biomedicines12071467.
3
The Impact of Sequential Therapies after First-Line Systemic Therapies in Unresectable Hepatocellular Carcinoma.一线全身治疗后序贯治疗对不可切除肝细胞癌的影响
J Clin Med. 2024 Apr 29;13(9):2612. doi: 10.3390/jcm13092612.
4
Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.2022 年全球癌症统计数据：全球 185 个国家和地区 36 种癌症的发病率和死亡率全球估计数。
CA Cancer J Clin. 2024 May-Jun;74(3):229-263. doi: 10.3322/caac.21834. Epub 2024 Apr 4.
5
Generating Patient-Derived HCC Cell Lines Suitable for Predictive In Vitro and In Vivo Drug Screening by Orthotopic Transplantation.通过原位移植生成适合预测体外和体内药物筛选的患者来源 HCC 细胞系。
Cells. 2023 Dec 30;13(1):82. doi: 10.3390/cells13010082.
6
T-Cell Exhaustion in HIV-1/Hepatitis C Virus Coinfection Is Reduced After Successful Treatment of Chronic Hepatitis C.慢性丙型肝炎成功治疗后，HIV-1/丙型肝炎病毒合并感染中的T细胞耗竭有所减轻。
Open Forum Infect Dis. 2023 Oct 21;10(11):ofad514. doi: 10.1093/ofid/ofad514. eCollection 2023 Nov.
7
Unleashing the efficacy of immune checkpoint inhibitors for advanced hepatocellular carcinoma: factors, strategies, and ongoing trials.释放免疫检查点抑制剂对晚期肝细胞癌的疗效：影响因素、策略及正在进行的试验
Front Pharmacol. 2023 Aug 31;14:1261575. doi: 10.3389/fphar.2023.1261575. eCollection 2023.
8
Functional significance of cholesterol metabolism in cancer: from threat to treatment.胆固醇代谢在癌症中的功能意义：从威胁到治疗。
Exp Mol Med. 2023 Sep;55(9):1982-1995. doi: 10.1038/s12276-023-01079-w. Epub 2023 Sep 1.
9
Dynamic changes of phenotype and function of natural killer cells in peripheral blood before and after thermal ablation of hepatitis B associated hepatocellular carcinoma and their correlation with tumor recurrence.热消融治疗乙型肝炎相关肝细胞癌前后外周血自然杀伤细胞表型和功能的动态变化及其与肿瘤复发的关系。
BMC Cancer. 2023 May 30;23(1):486. doi: 10.1186/s12885-023-10823-4.
10
3D cancer models: One step closer to human studies.3D 癌症模型：离人体研究更近一步。
Front Immunol. 2023 Apr 11;14:1175503. doi: 10.3389/fimmu.2023.1175503. eCollection 2023.