Orlic D, Kirk E, Quaini F
Exp Hematol. 1985 Sep;13(8):821-6.
In response to prolonged, intermittent exposure to hypoxia, the spleens of adult BALB/c mice displayed an initial increase and subsequent decrease in erythropoietic activity. The enzyme 2,5-adenylate synthetase was assayed during this period, and a direct relationship was found between the rate of red cell production and enzyme activity; that is, 2,5-adenylate synthetase activity was maximum in the spleen cell populations that contained the largest number of nucleated erythroid cells and minimum in those populations that contained the fewest nucleated erythroid cells. In contrast to this finding, synthetase activity was inversely related to the number of lymphocytes present in these spleen cell populations. On the basis of these observations, it appears that 2,5-adenylate synthetase is present in nucleated erythroid cells. If active in late erythroblasts, 2,5-adenylate synthetase may function as an inhibitor of DNA synthesis and/or hemoglobin synthesis.
成年BALB/c小鼠的脾脏在长期间歇性低氧暴露后，红细胞生成活性先升高后降低。在此期间对2,5-腺苷酸合成酶进行了检测，发现红细胞生成速率与酶活性之间存在直接关系；也就是说，在含有最多有核红细胞的脾细胞群体中，2,5-腺苷酸合成酶活性最高，而在含有最少有核红细胞的群体中活性最低。与这一发现相反，合成酶活性与这些脾细胞群体中淋巴细胞的数量呈负相关。基于这些观察结果，似乎2,5-腺苷酸合成酶存在于有核红细胞中。如果在晚幼红细胞中具有活性，2,5-腺苷酸合成酶可能作为DNA合成和/或血红蛋白合成的抑制剂发挥作用。
