Zhuravkin I N, Kozlov V A
Tsitologiia. 1982 Mar;24(3):340-4.
Polypotential hemopoietic stem cell numbers were monitored by means of the splenic colony-forming unit (CFU-S) method in bone marrow and spleen of CBA mice in condition of suppressed erythropoiesis (after syngeneic red blood cell hypertransfusion and chronic hypoxia). Erythropoietic activity determined by radioactive isotope 59Fe uptake in erythroid cells of spleen, bone marrow and erythrocytes of peripheral blood sharply decreased after these treatments. CFU-S contents in bone marrow and spleen increased during a week after syngeneic red blood cell hypertransfusion and after chronic hypoxia. It is assumed that the increase in hemopoietic stem cell contents in hemopoietic organs of mice under inhibited erythropoiesis may be interpreted due to the appearance of vacant differentiation potencies of stem cells, which in the normal state is realized in production of erythroid cells.
采用脾集落形成单位（CFU-S）法，在红细胞生成受抑制的条件下（同基因红细胞大量输注后和慢性缺氧后），监测CBA小鼠骨髓和脾脏中的多能造血干细胞数量。通过放射性同位素59Fe摄取来测定脾脏、骨髓的红系细胞以及外周血红细胞中的红细胞生成活性，在这些处理后急剧下降。同基因红细胞大量输注后以及慢性缺氧后一周内，骨髓和脾脏中的CFU-S含量增加。据推测，在红细胞生成受抑制的小鼠造血器官中，造血干细胞含量的增加可能是由于干细胞出现了空缺的分化潜能，而在正常状态下这种潜能体现在红系细胞的生成中。
