Cui Di, Yang Wenye, Guan Bo, Wu Wenxu, Yu Wenjiang
Fuyang Medical College, Fuyang Normal University, Fuyang 236037, Anhui, China.
Clinical Laboratory, Fuyang Minsheng Hospital, Fuyang 236072, Anhui, China.
Genet Res (Camb). 2025 May 19;2025:2237539. doi: 10.1155/genr/2237539. eCollection 2025.
Clear cell renal cell carcinoma (ccRCC) is the most common and highly malignant subtype of renal cancer, characterized by significant lipid deposition. Research has indicated that its growth and metastasis are closely associated with fatty acid metabolism. In this study, we integrated TCGA transcriptome data, CPTAC proteomics data, and the single-cell dataset GSE152938 to identify differentially expressed genes related to fatty acid metabolism in ccRCC. Using the LASSO algorithm, we constructed a prognostic model based on these genes. Western blot and PCR analyses confirmed the expression levels of the in ccRCC, while lentiviral transduction was used to investigate the effects of expression on tumor biological behaviors. Our findings demonstrated that expression is downregulated in ccRCC, and lower levels correlate with better patient prognosis. Functional assays showed that overexpression of significantly inhibited the proliferation and migration of ccRCC cells and increased their sensitivity to the chemotherapeutic drug oxaliplatin. This study highlights the potential tumor-suppressive role of in ccRCC and suggests its viability as a diagnostic and therapeutic target.
透明细胞肾细胞癌（ccRCC）是肾癌最常见且恶性程度高的亚型，其特征为大量脂质沉积。研究表明，其生长和转移与脂肪酸代谢密切相关。在本研究中，我们整合了TCGA转录组数据、CPTAC蛋白质组学数据以及单细胞数据集GSE152938，以鉴定ccRCC中与脂肪酸代谢相关的差异表达基因。使用LASSO算法，我们基于这些基因构建了一个预后模型。蛋白质印迹和PCR分析证实了ccRCC中[此处原文缺失相关基因名称]的表达水平，同时使用慢病毒转导来研究[此处原文缺失相关基因名称]表达对肿瘤生物学行为的影响。我们的研究结果表明，ccRCC中[此处原文缺失相关基因名称]的表达下调，且较低的[此处原文缺失相关基因名称]水平与患者较好的预后相关。功能分析表明，[此处原文缺失相关基因名称]的过表达显著抑制了ccRCC细胞的增殖和迁移，并增加了它们对化疗药物奥沙利铂的敏感性。本研究突出了[此处原文缺失相关基因名称]在ccRCC中的潜在肿瘤抑制作用，并表明其作为诊断和治疗靶点的可行性。
