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鉴定LIMK1作为透明细胞肾细胞癌的生物标志物：从数据挖掘到验证。

Identification of LIMK1 as a biomarker in clear cell renal cell carcinoma: from data mining to validation.

作者信息

Li Yifei, Fan Congcong, Jiang Feng, Zhang Jingnan, Li Yanzhen, Jiang Yanjie, Zhang Rui, Yu Zhixian, Wang Siqi

机构信息

Zhejiang Key Laboratory of Intelligent Cancer Biomarker Discovery and Translation, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.

Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

出版信息

J Cancer Res Clin Oncol. 2025 Mar 8;151(3):104. doi: 10.1007/s00432-025-06146-5.

DOI:10.1007/s00432-025-06146-5
PMID:40056237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11890329/
Abstract

PURPOSE

Clear cell renal cell carcinoma (ccRCC) is one of the most common types of renal cancer. LIM kinase 1 (LIMK1) reportedly plays an important role in tumorigenesis. However, the involvement of LIMK1 in the progression of ccRCC remains ambiguous.

METHODS

Based on the TCGA and CPTAC databases, the expression of LIMK1 in ccRCC was evaluated. In the TCGA-ccRCC cohort, the relationships between LIMK1 and immune cell infiltration as well as immune checkpoints were assessed. The high expression of LIMK1 in ccRCC was verified by qRT-PCR in four RCC cell lines. Immunohistochemistry was used to evaluate the expression of LIMK1 in clinical samples. The association between LIMK1 expression and survival prognosis was explored via Kaplan-Meier survival curve in the TCGA-ccRCC and local cohorts. The effects of LIMK1 knockdown on the proliferation, migration, and invasion abilities of RCC cells were evaluated via colony, CCK-8, wound healing, and Transwell assays.

RESULTS

Elevated expression level of LIMK1 was found in the TCGA-ccRCC cohort and was confirmed in RCC cell lines and clinical samples. Up-regulation of LIMK1 was found to be correlated with poor prognosis in TCGA-ccRCC and external cohorts. In addition, high-LIMK1 was associated with clinicopathological stage, immune cell infiltration and immune checkpoint in ccRCC. Importantly, knockdown of LIMK1 diminished the capability of proliferation, migration, and invasion in RCC cells.

CONCLUSION

LIMK1 may serve as a promising diagnostic and prognostic biomarker of ccRCC.

摘要

目的

透明细胞肾细胞癌（ccRCC）是最常见的肾癌类型之一。据报道，LIM激酶1（LIMK1）在肿瘤发生中起重要作用。然而，LIMK1在ccRCC进展中的作用仍不明确。

方法

基于TCGA和CPTAC数据库，评估LIMK1在ccRCC中的表达。在TCGA-ccRCC队列中，评估LIMK1与免疫细胞浸润以及免疫检查点之间的关系。通过qRT-PCR在四种RCC细胞系中验证LIMK1在ccRCC中的高表达。免疫组织化学用于评估临床样本中LIMK1的表达。通过TCGA-ccRCC和本地队列中的Kaplan-Meier生存曲线探索LIMK1表达与生存预后之间的关联。通过集落、CCK-8、伤口愈合和Transwell试验评估LIMK1敲低对RCC细胞增殖、迁移和侵袭能力的影响。

结果

在TCGA-ccRCC队列中发现LIMK1表达水平升高，并在RCC细胞系和临床样本中得到证实。发现LIMK1上调与TCGA-ccRCC和外部队列中的不良预后相关。此外，高LIMK1与ccRCC的临床病理分期、免疫细胞浸润和免疫检查点相关。重要的是，敲低LIMK1可降低RCC细胞的增殖、迁移和侵袭能力。

结论

LIMK1可能是ccRCC一种有前景的诊断和预后生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/4bfb75e9f9fd/432_2025_6146_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/6c1e5e8a565b/432_2025_6146_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/532af58841f4/432_2025_6146_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/34d01714efd9/432_2025_6146_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/45012a7ad3a5/432_2025_6146_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/4d8a36bbc9db/432_2025_6146_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/4bfb75e9f9fd/432_2025_6146_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/6c1e5e8a565b/432_2025_6146_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/47270719ce22/432_2025_6146_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/b7f0bc2fcdab/432_2025_6146_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/22114930ea17/432_2025_6146_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/532af58841f4/432_2025_6146_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/34d01714efd9/432_2025_6146_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/45012a7ad3a5/432_2025_6146_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/4d8a36bbc9db/432_2025_6146_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62fd/11890329/4bfb75e9f9fd/432_2025_6146_Fig9_HTML.jpg

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Integrated genomic analysis reveals aberrations in WNT signaling in germ cell tumors of childhood and adolescence.整合基因组分析揭示了儿童和青少年生殖细胞肿瘤中 WNT 信号通路的异常。
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