埃及薰衣草叶、茎和果实提取物的靶向代谢组学分析及抗菌评估

Targeted metabolomic profiling and antibacterial assessment of extracts from leaves, stems, and fruits of Egyptian L.

作者信息

Abd Elkarim Asmaa S, Medhat Tulip A, Essa Ahmed F, Abdelaziz Sahar, Hafez Samia S

机构信息

Chemistry of Tanning Materials and Leather Technology Department, National Research Centre Giza 12622 Egypt

Obour Health Insurance Organization Clinics Obour City Egypt.

出版信息

RSC Adv. 2025 May 27;15(22):17442-17465. doi: 10.1039/d5ra00906e. eCollection 2025 May 21.

Abstract

This study investigated the metabolic profiles and antibacterial activities of leaves, stems, and fruits. Butanol extracts were analyzed using LC-ESI-MS/MS, tentatively identifying 116 secondary metabolites based on fragmentation patterns, biosynthetic pathways, and literature comparisons. Spectral similarity networks generated through the Global Natural Products Social Network (GNPS) revealed chemical similarities and identified 6 uncommon flavone compounds. Spectral similarity analysis revealed a close chemical resemblance between leaves and stems, while fruits exhibited distinct profiles. Antibacterial activity was assessed against seven pathogenic strains using both disk diffusion and microbroth dilution methods. Leaf extracts demonstrated the strongest activity, with inhibition zones up to 20.13 mm and MIC values as low as 1.5 mg mL, particularly against ATCC 29213. Stem extracts showed comparable efficacy, while fruit extracts were more effective against These findings highlight as a promising natural source of bioactive compounds for potential antimicrobial applications.

摘要

本研究调查了叶、茎和果实的代谢谱及抗菌活性。使用液相色谱-电喷雾串联质谱(LC-ESI-MS/MS)对丁醇提取物进行分析,根据碎片模式、生物合成途径和文献比较,初步鉴定出116种次生代谢产物。通过全球天然产物社会网络(GNPS)生成的光谱相似性网络揭示了化学相似性,并鉴定出6种罕见的黄酮类化合物。光谱相似性分析表明叶和茎之间存在密切的化学相似性,而果实呈现出独特的谱图。使用纸片扩散法和微量肉汤稀释法对七种致病菌株进行了抗菌活性评估。叶提取物表现出最强的活性,抑菌圈高达20.13毫米,最低抑菌浓度(MIC)值低至1.5毫克/毫升,尤其对ATCC 29213有效。茎提取物显示出相当的疗效,而果实提取物对[此处原文缺失具体菌株]更有效。这些发现突出了[此处原文缺失具体内容]作为潜在抗菌应用中生物活性化合物的有前景的天然来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85d0/12107345/52092b17552a/d5ra00906e-f1.jpg

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