Zhang Jing, Zha Min, Xiao Shanghua, Filipczak Nina, Yalamarty Satya Siva Kishan, Wu Xiangping, Gong Chenkai, Li Xiang
Key Laboratory of Modern Preparation of TCM, Ministry of Education, National Key Laboratory for the Modernization of Classical and Famous Prescriptions of Chinese Medicine, National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, 330004, People's Republic of China.
Jiangzhong Pharmaceutical Co. Ltd, Nanchang, Jiangxi, 330049, People's Republic of China.
Int J Nanomedicine. 2025 May 22;20:6515-6531. doi: 10.2147/IJN.S510514. eCollection 2025.
Nanoparticles constructed with silver/manganese dioxide (Ag@MnO) as the core, in conjunction with survivin siRNA (sis) and cyclo(RGD-DPhe-K) (Ag@MnO-sis-c-L), were prepared for integrated tumor diagnosis and therapy.
Ag@MnO-sis-c-L particles were prepared and characterized. The silver and manganese content were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES). The stability of sis in the system was evaluated by incubation with 50% FBS before the agarose gel electrophoresis experiment. The in vitro photothermal conversion ability, cytotoxicity to 4T1 cells, and cellular uptake of preparations were evaluated. The dialysis technique was employed to determine the in vitro release profile of Ag and Mn from Ag@MnO-sis-c-L under various pH conditions. The pharmacokinetic behavior and tissue distribution of silver in vivo were detected by ICP-OES. Animal model experiments were conducted to further evaluate the anti-tumor efficacy of Ag@MnO-sis-c-L against breast cancer in combination with infrared irradiation.
Our newly synthesized Ag@MnO-sis-c-L nanoparticles displayed superior physicochemical properties. The combined application of these nanoparticles with photothermal therapy (PTT) exerted the strongest synergistic inhibitory effects on tumor growth. Survivin protein expression in tumor tissues were markedly suppressed following delivery of nanoparticles loaded with sis. Additionally, magnetic resonance imaging revealed the high imaging capability of hybrid nanoparticles.
This study supports the potential utility of Ag@MnO-sis-c-L coupled with PTT in therapeutic and diagnostic imaging applications.
制备以银/二氧化锰（Ag@MnO）为核心、联合生存素小干扰RNA（sis）和环（RGD-DPhe-K）（Ag@MnO-sis-c-L）构建的纳米颗粒，用于肿瘤的一体化诊断与治疗。
制备并表征Ag@MnO-sis-c-L颗粒。通过电感耦合等离子体发射光谱法（ICP-OES）测定银和锰的含量。在琼脂糖凝胶电泳实验前，通过与50%胎牛血清孵育来评估sis在体系中的稳定性。评估制剂的体外光热转换能力、对4T1细胞的细胞毒性以及细胞摄取情况。采用透析技术测定在不同pH条件下Ag@MnO-sis-c-L中Ag和Mn的体外释放曲线。通过ICP-OES检测体内银的药代动力学行为和组织分布。进行动物模型实验，以进一步评估Ag@MnO-sis-c-L联合红外照射对乳腺癌的抗肿瘤疗效。
我们新合成的Ag@MnO-sis-c-L纳米颗粒表现出优异的理化性质。这些纳米颗粒与光热疗法（PTT）联合应用对肿瘤生长发挥了最强的协同抑制作用。负载sis的纳米颗粒递送后，肿瘤组织中生存素蛋白表达明显受到抑制。此外，磁共振成像显示了杂化纳米颗粒的高成像能力。
本研究支持Ag@MnO-sis-c-L联合PTT在治疗和诊断成像应用中的潜在效用。
