Hemamalini Dileepkumar, Sundari S Shantha, Faizee K M Shahul Hameed, Jeyachandran Sivakamavalli
Department of Orthodontics and Dentofacial Orthopedics, Saveetha Dental college, Saveetha Institute of medical and technical science (SIMATS)Saveetha University, Chennai, Tamilnadu, India.
Department of Orthodontics and Dentofacial Orthopedics, Sathayabama Dental college and Hospital, Sathyabama Institute of science and technology, Sathyabama University, Chennai, Tamilnadu, India.
Biomater Investig Dent. 2025 May 14;12:43612. doi: 10.2340/biid.v12.43612. eCollection 2025.
This study aimed to evaluate the antibacterial, antibiofilm, antioxidant and anti-inflammatory properties and of extracts.
The sample was washed and extracted using methanol. The mixture was homogenized using a blender and centrifuged at high speed (10,000 × g) for 2 min, then stirred at room temperature for 30 min using magnetic stirrer, to ensure thorough extraction. Afterward, it was centrifuged at 5,000 × g for 10 min to separate the dissolved components from undissolved debris. Following this antioxidant activity was assessed using DPHH assay, the antimicrobial effects were tested against , , and using Kirby-Bauer disk diffusion, biofilm inhibition assay was done to assess biofilm inhibition against , , and . Finally, the anti-inflammatory activities of the were determined using a modified version of the BSA assay.
When tested against , , , and strains, the antimicrobial evaluation revealed that the extract successfully inhibited biofilm formation when tested against the same organism, and it also demonstrated increased activity with increasing concentration. The zone of inhibition progressively expanded with increasing concentration, reaching a maximum of 17 mm ± 0.1 for 100 µg/mL. In terms of antioxidant activity, the metholic extract gradually increased from 10 µg/mL to a higher activity at 40 µg/mL in comparison to the control and blank, and then decreased at a dose of 50 µg/mL. At different doses, the anti-inflammatory action of extracts successfully inhibited BSA denaturation, which causes inflammation; the maximum activity has been observed.
This comprehensive analysis highlights as a valuable natural resource with multifaceted biological activities, supporting its further investigation for therapeutic applications in dentistry.
本研究旨在评估提取物的抗菌、抗生物膜、抗氧化和抗炎特性。
将样品洗涤后用甲醇提取。用搅拌机将混合物匀浆，然后以10000×g的高速离心2分钟，接着在室温下用磁力搅拌器搅拌30分钟，以确保充分提取。之后，以5000×g离心10分钟，将溶解成分与未溶解的残渣分离。随后，使用DPHH法评估抗氧化活性，采用 Kirby-Bauer 纸片扩散法测试对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌的抗菌效果，进行生物膜抑制试验以评估对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌的生物膜抑制作用。最后，使用改良版的 BSA 试验测定提取物的抗炎活性。
在针对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌菌株进行测试时，抗菌评估显示，该提取物在针对相同生物体进行测试时成功抑制了生物膜形成，并且随着浓度增加其活性也增强。抑菌圈随着浓度增加而逐渐扩大，100μg/mL时达到最大，为17mm±0.1。在抗氧化活性方面，与对照和空白相比，该甲醇提取物的抗氧化活性从10μg/mL逐渐增加至40μg/mL时活性更高，然后在50μg/mL剂量时下降。在不同剂量下，提取物的抗炎作用成功抑制了导致炎症的BSA变性；已观察到最大活性。
这项综合分析突出了[提取物名称未明确]作为一种具有多方面生物活性的宝贵天然资源，支持对其在牙科治疗应用方面的进一步研究。
