Centre for Natural Products & Traditional Knowledge, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India; Analytical Chemistry, CSIR-Indian Institute of Chemical Technology, Hyderabad 500 007, India.
Food Res Int. 2022 Jul;157:111394. doi: 10.1016/j.foodres.2022.111394. Epub 2022 May 23.
Marine seaweeds are predominant for nutraceuticals and have been food source since ancient times and are currently being used in Chinese medicines and Japanese traditional medicines. In the present study, the chemical profile analysis of Halimeda gracilis was performed using UPLC-ESI-Q-TOF-MS analysis and assessed for its anticancer activity. A cursory investigation of the total ion chromatograms of the both methanol (MHG) and ethyl acetate (EAHG) of H. gracilis extracts reveals that both extracts have different kind of metabolites including phenols and its derivatives, diterpenes, cinnamic acids derivatives etc. The in vitro anticancer activity of EAHG and MHG exhibiting significant activity and induced apoptosis against skin cancer cells by generating excessive ROS, damaging the mitochondrial membrane and nuclear components. Further, the western blot analysis showed that the EAHG and MHG downregulates the oncoproteins PI3K, AKT, p-AKT, and BcL2 and upregulates the apoptotic proteins Bax, Cyto C, p21, p53, Caspase 9 and Caspase 3. To best of our knowledge, this is the first report which implies the UPLC-ESI-Q-TOF-MS based chemical profiling of H. gracilis and can be used for the treatment of cancer.
海洋藻类是营养保健品的主要来源，自古以来就是食物来源，目前被用于中药和日本传统药物中。在本研究中，使用 UPLC-ESI-Q-TOF-MS 分析对石莼的化学成分进行了分析，并评估了其抗癌活性。对石莼甲醇（MHG）和乙酸乙酯（EAHG）提取物的总离子色谱图进行初步研究表明，两种提取物都含有不同种类的代谢物，包括酚类及其衍生物、二萜、肉桂酸衍生物等。EAHG 和 MHG 的体外抗癌活性显示出显著的活性，通过产生过量的 ROS、破坏线粒体膜和核成分，诱导皮肤癌细胞凋亡。此外，Western blot 分析表明，EAHG 和 MHG 下调癌蛋白 PI3K、AKT、p-AKT 和 BcL2，并上调凋亡蛋白 Bax、Cyto C、p21、p53、Caspase 9 和 Caspase 3。据我们所知，这是首次基于 UPLC-ESI-Q-TOF-MS 对石莼进行化学成分分析的报告，可用于癌症治疗。
