Abbas Zainab Khamis, Hilo Zainab Qassim Mohammed, Hussein Wafia Shaker Abdul, Mohsein Osama A
Department of Microbiology, College of Medicine, Anbar University, Anbar, Iraq.
University of Babylon, DNA Research Center, Babylon, Iraq.
Trop Parasitol. 2025 Jan-Jun;15(1):33-41. doi: 10.4103/tp.tp_57_24. Epub 2025 Apr 5.
Different kinds of leishmaniasis can be distinguished based on the range and complexity of clinical presentations, which can vary from asymptomatic infection to severe illness that greatly affects one's quality of life.
Determining the relationship between inflammatory cytokines and the severity of leishmaniasis.
A case-control study comprising 150 patients diagnosed with cutaneous leishmaniasis (75 males and 75 females) with 50 healthy controls. The individuals in question ranged in age from 35 to 45. The study was carried out at Nasiriyah General Hospital and Al-Habobbi Teaching Hospital between January 1, 2024, and August 1, 2024. Inclusion criteria required patients aged 35-45 years, with clinically confirmed leishmaniasis and positive serological results, who provided consent for participation. Exclusion criteria included individuals with immunological disorders and pregnant people. The infected group was clinically evaluated to exclude other infections or inflammatory conditions that could affect cytokine levels. Patients were categorized into mild, moderate, and severe leishmaniasis based on clinical criteria, including lesion size, number, complications, and infection duration, following established guidelines. The serum was isolated using centrifugation and preserved at a temperature of -20°C to quantify the concentrations of anti- antibodies, interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), and CRP using the enzyme-linked immunosorbent assay (ELISA) method. The ELISA kits used to measure cytokine levels and antibody titers were sourced from Bio-Techne, an American company.
The results showed nonstatistically significant differences in mean age or gender distribution between patients and healthy controls. However, fever was more common in patients, and the severity of the disease was classified into three grades. Levels of antibodies, IFN-γ, tumor necrosis factor, IL-10, and C-reactive protein were also significantly higher in patients than in healthy controls, suggesting an association with leishmaniasis infection.
The results confirm the relationship between levels of antibodies and immune complexes, such as IFN-γ and TNF-α, with the severity of leishmaniasis infection. These high levels indicate an intense immune response, reflecting the body's inflammatory reaction to the infection.
不同类型的利什曼病可根据临床表现的范围和复杂性加以区分，临床表现的范围可从无症状感染到严重疾病，严重疾病会极大影响生活质量。
确定炎性细胞因子与利什曼病严重程度之间的关系。
一项病例对照研究，纳入150例诊断为皮肤利什曼病的患者（75例男性和75例女性）以及50名健康对照者。研究对象年龄在35岁至45岁之间。该研究于2024年1月1日至2024年8月1日在纳西里耶综合医院和哈博比教学医院开展。纳入标准要求患者年龄在35 - 45岁之间，临床确诊为利什曼病且血清学结果呈阳性，并同意参与研究。排除标准包括患有免疫性疾病的个体和孕妇。对感染组进行临床评估，以排除可能影响细胞因子水平的其他感染或炎症性疾病。根据既定指南，依据病变大小、数量、并发症和感染持续时间等临床标准，将患者分为轻度、中度和重度利什曼病。采用离心法分离血清，并保存在-20°C的温度下，使用酶联免疫吸附测定（ELISA）法对抗体、干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）和C反应蛋白（CRP）的浓度进行定量。用于测量细胞因子水平和抗体滴度的ELISA试剂盒购自美国公司Bio-Techne。
结果显示，患者与健康对照者之间的平均年龄或性别分布无统计学显著差异。然而，发热在患者中更为常见，且疾病严重程度分为三个等级。患者体内的抗体、IFN-γ、肿瘤坏死因子、IL-10和C反应蛋白水平也显著高于健康对照者，表明这些指标与利什曼病感染有关。
结果证实了抗体和免疫复合物（如IFN-γ和TNF-α）水平与利什曼病感染严重程度之间的关系。这些高水平表明存在强烈的免疫反应，反映了机体对感染的炎症反应。
