哺乳动物唾液腺和唾液中存在血管活性肠肽-促胰液素家族的类海蜥蜴毒素肽。

Presence of helodermin-like peptides of the VIP-secretin family in mammalian salivary glands and saliva.

作者信息

Robberecht P, De Neef P, Vandermeers A, Vandermeers-Piret M C, Svoboda M, Meuris S, De Graef J, Woussen-Colle M C, Yanaihara C, Yanaihara N

出版信息

FEBS Lett. 1985 Oct 7;190(1):142-6. doi: 10.1016/0014-5793(85)80445-2.

DOI:10.1016/0014-5793(85)80445-2

PMID:4043392

Abstract

Helodermin is a biologically active peptide isolated from the venom of the Gila monster lizard (Heloderma suspectum) whose structure is related to that of vasoactive intestinal peptide and secretin. Using a specific radioimmunoassay based on antisera prepared by immunizing rabbits with natural helodermin, we demonstrated the presence of helodermin-like material in mammalian salivary glands, including parotid, submaxillary and sublingual glands from rat and dog, and parotid and submaxillary glands from man. All helodermin-like materials had an apparent molecular mass of 4-12 kDa. Dog saliva, collected after pilocarpine stimulation, revealed similar immunoreactivity with a major component around 6 kDa.

摘要

蛙皮素是从希拉毒蜥（Heloderma suspectum）毒液中分离出的一种生物活性肽，其结构与血管活性肠肽和促胰液素相关。我们采用基于用天然蛙皮素免疫兔子制备的抗血清的特异性放射免疫分析法，证实在哺乳动物唾液腺中存在类似蛙皮素的物质，包括大鼠和狗的腮腺、颌下腺和舌下腺，以及人类的腮腺和颌下腺。所有类似蛙皮素的物质的表观分子量为4 - 12 kDa。毛果芸香碱刺激后收集的狗唾液显示出类似的免疫反应性，主要成分约为6 kDa。