Sundler F, Christophe J, Robberecht P, Yanaihara N, Yanaihara C, Grunditz T, Håkanson R
Department of Medical Cell Research, University of Lund, Sweden.
Regul Pept. 1988 Jan;20(1):83-9. doi: 10.1016/0167-0115(88)90060-2.
Helodermin is a VIP/secretin-like 35-amino acid peptide originally isolated from the venom of the lizard Gila monster. Recently, helodermin-immunoreactive material was demonstrated in mammalian salivary glands, brain and gut. In the present study 8 human medullary thyroid carcinomas as well as 4 normal thyroid glands were examined immunocytochemically for the presence of helodermin using an antiserum raised against helodermin-(5-35) that does not cross-react with VIP or secretin. Cells displaying helodermin-like immunoreactivity were found in all tumours examined except one. On the whole the helodermin-immunoreactive cells had the same distribution as those storing calcitonin, suggesting coexistence of the two peptides in most of the tumour cells. Also normal human C-cells displayed helodermin immunoreactivity. The results suggest that a peptide chemically related to helodermin is a constituent of human medullary thyroid carcinoma cells as well as of normal C-cells.
海洛德明是一种与血管活性肠肽/促胰液素类似的由35个氨基酸组成的肽，最初是从毒蜥的毒液中分离出来的。最近，在哺乳动物的唾液腺、大脑和肠道中发现了海洛德明免疫反应性物质。在本研究中，使用针对海洛德明-（5-35）产生的抗血清，对8例人甲状腺髓样癌以及4例正常甲状腺进行了免疫细胞化学检查，以检测海洛德明的存在，该抗血清与血管活性肠肽或促胰液素无交叉反应。除1例肿瘤外，在所有检查的肿瘤中均发现了显示出海洛德明样免疫反应性的细胞。总体而言，海洛德明免疫反应性细胞的分布与储存降钙素的细胞相同，这表明在大多数肿瘤细胞中这两种肽共存。正常人类C细胞也显示出海洛德明免疫反应性。结果表明，一种与海洛德明化学相关的肽是人类甲状腺髓样癌细胞以及正常C细胞的组成成分。
