Dale M M, Obianime W
FEBS Lett. 1985 Oct 7;190(1):6-10. doi: 10.1016/0014-5793(85)80415-4.
The effect of phorbol myristate acetate (PMA) was compared with that of histamine on the guinea-pig lung parenchymal strip. PMA, 10(-5) M, caused a slowly developing sustained contraction which had approximately the same magnitude as the maximal histamine contraction. Isoprenaline, at 10(-5) M, caused 86% relaxation of the histamine contraction but only 22% relaxation of the PMA contraction. Forskolin, at 10(-5) M had a similar action to isoprenaline on the effects of both spasmogens while aminophylline, 5 X 10(-4) M, was considerably less effective. Sodium nitroprusside had little effect on the histamine contraction and actually increased the PMA spasm. It is suggested that protein kinase C may have a role in the tonic phase of the contraction of bronchiolar smooth muscle. These findings could have relevance for the delayed phase of asthma, which is known to be insensitive to beta-agonists.
将佛波醇肉豆蔻酸酯乙酸酯（PMA）与组胺对豚鼠肺实质条的作用进行了比较。10(-5) M的PMA引起缓慢发展的持续收缩，其幅度与组胺最大收缩幅度大致相同。10(-5) M的异丙肾上腺素可使组胺收缩松弛86%，但仅使PMA收缩松弛22%。10(-5) M的福斯高林对两种致痉剂的作用与异丙肾上腺素相似，而5×10(-4) M的氨茶碱效果则要差得多。硝普钠对组胺收缩作用不大，实际上还增强了PMA痉挛。提示蛋白激酶C可能在细支气管平滑肌收缩的紧张期起作用。这些发现可能与哮喘的迟发相有关，已知哮喘迟发相对β-激动剂不敏感。
