Yang Qing, Jin Wenhui, Fang Hua, Chen Weizhu, Xie Quanling, Chen Hui, Liu Qian, Jiang Xian, Wang Shaohua, Zhang Longtao, Zhang Yiping, Hong Zhuan
Technical Innovation Center for Utilization of Marine Biological Resources, Ministry of Natural Resources, Third Institute of Oceanography, Xiamen, 361005, China.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Bioprocess Biosyst Eng. 2025 May 28. doi: 10.1007/s00449-025-03177-7.
Enhancing the stability and the reusability of ulva polysaccharide lyase (UPL) is crucial for the efficient production of reducing sugars from ulva polysaccharides, which are vital for their broad applications in functional foods. In this study, we innovatively developed a self-immobilized UPL by fusing the enzyme with ferritin, leading to the spontaneous formation of micron-sized ulva polysaccharide lyase supraparticles (mUPLSPs). This novel system streamlines the enzyme purification and immobilization process into a single step, effectively circumventing the need for conventional, laborious chromatographic methods. The mUPLSPs exhibited superior stability and reusability, maintaining over 80% of their initial activity after five cycles of use. When compared to free UPLs, mUPLSPs displayed enhanced thermal and pH stability, resulting in a 252% increase in the yield of reducing sugars after a 40-hour reaction period. The ferritin-tagged, self-immobilization strategy not only provides a scalable and cost-efficient approach to the sustainable production of reducing sugars from ulva polysaccharides but also holds significant potential for industrial-scale applications.
提高石莼多糖裂解酶（UPL）的稳定性和可重复使用性对于从石莼多糖高效生产还原糖至关重要，还原糖在功能性食品中的广泛应用具有重要意义。在本研究中，我们创新性地通过将该酶与铁蛋白融合开发了一种自固定化UPL，导致微米级石莼多糖裂解酶超颗粒（mUPLSPs）的自发形成。这种新型系统将酶的纯化和固定化过程简化为一步，有效避免了传统繁琐的色谱方法的需求。mUPLSPs表现出卓越的稳定性和可重复使用性，在使用五个循环后仍保持其初始活性的80%以上。与游离UPL相比，mUPLSPs表现出增强的热稳定性和pH稳定性，在40小时的反应期后还原糖产量增加了252%。铁蛋白标记的自固定化策略不仅为从石莼多糖可持续生产还原糖提供了一种可扩展且经济高效的方法，而且在工业规模应用中具有巨大潜力。
