Weinberg S R, Gruber D F, Exum E D, Ledney G D
Exp Cell Biol. 1985;53(5):252-9. doi: 10.1159/000163319.
Hemopoietic changes in male C57BL/6Cum BR mice engrafted with Lewis lung carcinoma (3LL) were evaluated between day 7, when palpable tumors were present, to day 30 postengraftment. All experimental animals demonstrated decreasing hematocrits (down 40% by day 30) with concurrent leukocytosis which by day 30 postengraftment had reached levels 13.4 times normal. The myelocytic/erythrocytic ratio for normal animals was 1:3 (bone marrow: spleen). The ratio for engrafted animals ranged between 10:1 and 40:1. This apparent shift in production priorities is even more significant in light of the fact that femoral bone marrow cellularity had decreased by 33% on day 17. Splenomegaly, evident by day 7, was seven times control by day 17. Clonogenic analysis of erythroprogenitor cell concentrations revealed an inverse relationship between bone marrow and spleen. 27 days after engraftment, splenic populations demonstrated significant increases in colony forming unit-erythroid (115-fold), burst forming unit-erythroid (7.4-fold), whereas bone marrow concentrations had decreased (6-fold). This report suggests that initiation of 3LL tumor in mice results in a change in the degree of hematopoietic priorities and participation of erythroid organs.
对移植了Lewis肺癌（3LL）的雄性C57BL/6Cum BR小鼠的造血变化进行了评估，评估时间从出现可触及肿瘤的第7天到移植后第30天。所有实验动物均出现血细胞比容下降（到第30天时下降了40%），同时伴有白细胞增多，到移植后第30天时白细胞水平达到正常的13.4倍。正常动物的骨髓细胞/红细胞比例为1:3（骨髓：脾脏）。移植动物的该比例在10:1至40:1之间。鉴于在第17天时股骨骨髓细胞数量减少了33%，这种生产优先级的明显转变更为显著。脾脏肿大在第7天时明显可见，到第17天时是对照的7倍。对红系祖细胞浓度的克隆形成分析显示骨髓和脾脏之间呈负相关。移植后27天，脾脏群体的红系集落形成单位（增加了115倍）、红系爆式集落形成单位（增加了7.4倍）显著增加，而骨髓浓度则下降了（6倍）。本报告表明，小鼠中3LL肿瘤的起始导致造血优先级程度的改变以及红系器官的参与情况发生变化。
