Low K, Harbour C
Dev Biol Stand. 1985;60:73-9.
Commercial media used for the growth of hybridoma cells contain different glucose concentrations. We have attempted to establish the optimal levels of glucose required for maximum hybridoma cell yields by studying the energy source metabolism of two murine hybridoma cell lines in several commercial media in static 25 cm2 flask cultures. The glucose and lactate quotients and growth yields from glucose of the two cell lines were similar in all media. Glucose limited growth in DME containing lg/L. Glucose supplementation to 2g/L in DME significantly increased cell and antibody yields. When fructose was used as a substitute for glucose the fructose quotient of one cell line was found to be similar to its glucose quotient whereas that of the other cell line was significantly reduced compared to its glucose quotient.
用于杂交瘤细胞生长的商业培养基含有不同的葡萄糖浓度。我们试图通过研究两种小鼠杂交瘤细胞系在静态25平方厘米培养瓶中几种商业培养基中的能量源代谢，来确定获得最大杂交瘤细胞产量所需的最佳葡萄糖水平。在所有培养基中，这两种细胞系的葡萄糖和乳酸商以及葡萄糖的生长产量相似。在含有1g/L葡萄糖的杜氏改良Eagle培养基（DME）中，葡萄糖限制了细胞生长。在DME中添加葡萄糖至2g/L可显著提高细胞和抗体产量。当用果糖替代葡萄糖时，发现一种细胞系的果糖商与其葡萄糖商相似，而另一种细胞系的果糖商与其葡萄糖商相比则显著降低。
