Mahadevan M D, Klimkowsky J A, Deo Y M
Centocor, Inc., Malvern, Pennsylvania 19355.
Cytotechnology. 1994;14(2):89-96. doi: 10.1007/BF00758173.
A simple method to analyze media consumption and by-product build-up in high-cell-density perfusion cultures has been developed. This technique makes use of a media replenishment strategy which can be used to decouple the two phenomena. Also, the replenished media method can be used to identify limitation by complex and undefined media components such as bovine serum.
已开发出一种用于分析高细胞密度灌注培养中培养基消耗和副产物积累的简单方法。该技术采用了一种培养基补充策略，可用于将这两种现象解耦。此外，补充培养基的方法可用于识别复杂和未定义的培养基成分（如牛血清）造成的限制。
