Neto Sebastião Venancio, Azambuja Matheus, Nascimento Viviane Demetrio, Nogaroto Viviane, Vicari Marcelo Ricardo, Noleto Rafael Bueno
Departamento de Genética, Programa de Pós-Graduação em Genética, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, Paraná, Brazil.
Genome. 2025 Jan 1;68:1-9. doi: 10.1139/gen-2025-0004.
Transposable elements play an important role in determining the size and structure of eukaryotic genomes. Represented by several families, the superfamily is widely distributed in animal and plant genomes, and its structure has been characterized. is a Neotropical genus of treefrogs, and despite the frequent 2 = 24 chromosomes found in its representatives, the karyotypic organization of the species cannot be considered conserved due to the scarcity of studies focusing on chromosomal mapping of repetitive DNA sequences. Here, elements were isolated and mapped on the chromosomes of three species, followed by structural and phylogenetic analysis. The physical mapping revealed dispersed signals in euchromatin with small accumulations in some heterochromatic regions. All / transposons isolated in this study presented high sequence integrity, suggesting that these elements had a recent invasion phase and are active in the host genomes of these frog species. and presented a DD36E signature, while showed a new DD37E signature with a similar organizational structure and a close relationship with the known DD36E/ family. These findings improve our understanding of the diversity of / transposons and their role in the evolution of the hylid frog genome and karyotype.
转座元件在决定真核生物基因组的大小和结构方面发挥着重要作用。该超家族由几个家族代表，广泛分布于动植物基因组中，其结构已得到表征。 是树蛙的一个新热带属，尽管在其代表物种中经常发现2n = 24条染色体，但由于专注于重复DNA序列染色体定位的研究稀缺，该物种的核型组织不能被认为是保守的。在这里， 元件被分离并定位在三个 物种的染色体上，随后进行了结构和系统发育分析。物理图谱显示常染色质中有分散的信号，在一些异染色质区域有少量积累。本研究中分离出的所有 / 转座子都具有高度的序列完整性，这表明这些元件最近处于入侵阶段，并且在这些蛙类物种的宿主基因组中活跃。 和 呈现出DD36E特征，而 显示出一种新的DD37E特征，具有相似的组织结构，并且与已知的DD36E/ 家族关系密切。这些发现增进了我们对 / 转座子多样性及其在雨蛙科蛙类基因组和核型进化中作用的理解。
