Molecular characterization and chromosomal distribution of transposons in species (Anura, Hylidae).

Transposable elements play an important role in determining the size and structure of eukaryotic genomes. Represented by several families, the superfamily is widely distributed in animal and plant genomes, and its structure has been characterized. is a Neotropical genus of treefrogs, and despite the frequent 2 = 24 chromosomes found in its representatives, the karyotypic organization of the species cannot be considered conserved due to the scarcity of studies focusing on chromosomal mapping of repetitive DNA sequences. Here, elements were isolated and mapped on the chromosomes of three species, followed by structural and phylogenetic analysis. The physical mapping revealed dispersed signals in euchromatin with small accumulations in some heterochromatic regions. All / transposons isolated in this study presented high sequence integrity, suggesting that these elements had a recent invasion phase and are active in the host genomes of these frog species. and presented a DD36E signature, while showed a new DD37E signature with a similar organizational structure and a close relationship with the known DD36E/ family. These findings improve our understanding of the diversity of / transposons and their role in the evolution of the hylid frog genome and karyotype.