Zhang Yi, Wu Yuhao, Sun Jiayi, Su Yu, Li Xiaoyang, Jiang Hu, Guo Liang, Xiong Yonghua, Huang Xiaolin
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Nanchang University, Nanchang 330047, PR China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, PR China.
Food Chem. 2025 Oct 1;488:144898. doi: 10.1016/j.foodchem.2025.144898. Epub 2025 May 23.
Herein we present a double-color lateral flow immunoassay (D-MANP-LFIA) test strip for the rapid and simultaneous quantitative detection of fumonisin B1 (FB1) and T-2 toxin (T-2). The D-MANP-LFIA employs two differently colored core-shell structured magnetic aggregation-induced emission bifunctional nanoparticles (MANPs) as signal amplification tags. Leveraging their high fluorescence intensity and well-retained magnetic properties, the D-MANP-LFIA enables accurate and concurrent quantification of FB1 and T-2 through a chicken IgY antibody-mediated, independent C-line-assisted ratiometric signal output strategy. The detection limits for FB1 and T-2 were as low as 0.183 ng/mL and 0.017 ng/mL in PBS. Aditionally, the D-MANP-LFIA demonstrated good practicality, accurately quantifying FB1 and T-2 in feed and corn samples. Collectively, the D-MANP-LFIA provides a powerful tool for the rapid detection of multiple trace mycotoxins in complex matrices, making it highly valuable for food and feed safety monitoring.
在此，我们展示了一种用于快速同时定量检测伏马菌素B1（FB1）和T-2毒素（T-2）的双色侧向流动免疫分析（D-MANP-LFIA）试纸条。D-MANP-LFIA采用两种不同颜色的核壳结构磁性聚集诱导发光双功能纳米颗粒（MANPs）作为信号放大标签。利用其高荧光强度和良好保留的磁性，D-MANP-LFIA通过鸡IgY抗体介导的、独立的C线辅助比率信号输出策略，能够对FB1和T-2进行准确且同时的定量。在磷酸盐缓冲液（PBS）中，FB1和T-2的检测限低至0.183 ng/mL和0.017 ng/mL。此外，D-MANP-LFIA表现出良好的实用性，能够准确地对饲料和玉米样品中的FB1和T-2进行定量。总体而言，D-MANP-LFIA为复杂基质中多种痕量霉菌毒素的快速检测提供了一个强大的工具，使其在食品和饲料安全监测方面具有很高的价值。
