Hu Xueyan, Zhang Zhilong, Li Qiannan, Du Fangfang, Wang Xuebing, Yang Mingfan, Mao Sai, Zhang Hongying
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou city, Henan, PR China.
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou city, Henan, PR China; Key Laboratory for Animal-Derived Food Safety of Henan Province, Zhengzhou city, Henan, PR China; Key Laboratory for Study and Evaluation of Chinese Veterinary Medicine, Zhengzhou city, Henan, PR China.
Microb Pathog. 2025 Sep;206:107732. doi: 10.1016/j.micpath.2025.107732. Epub 2025 May 26.
Porcine Reproductive and Respiratory Syndrome (PRRS) is a severe infectious disease caused by the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). The prevalence of PRRSV worldwide significantly impacts the development of the swine industry, exerting a considerable adverse effect. PRRSV infection typically induces a substantial increase in pro-inflammatory factors within the host organism, such as IL-1β, IL-6, and TNF-α, resulting in an "inflammatory factor storm" that leads to lung lesions and severe viremia in pigs. Currently, there is no effective treatment available for this disease. Recent research indicates that Chinese herbal medicine demonstrates notable antiviral effects. In this study, we found that Isatis Root Polysaccharides (IRPS) can significantly inhibit PRRSV proliferation in vitro. To investigate the underlying cause of this phenomenon, modified porcine alveolar macrophage 3D4/21/CD163 cells were used as the target cells to study the inhibitory effect of IRPS on PRRSV-BJ4. This study demonstrates that IRPS exhibits direct virucidal activity against PRRSV and significantly inhibits the invasion of the PRRSV-BJ4 strain. IFA, ELISA, or Western blot techniques were employed to evaluate and confirm PRRSV proliferation, cytokine secretion, or TLR4 transcript and protein levels. The studies have revealed that IRPS affects cytokine secretion by influencing TLR4 and its downstream inflammatory signaling pathways, thereby preventing the development of the inflammatory factor storm and the subsequent infection of PRRSV. In this experiment, we observed a significant inhibitory effect of IRPS on the proliferation of PRRSV in vitro. This finding establishes a theoretical foundation for further research on the antiviral properties of traditional Chinese medicine.
猪繁殖与呼吸综合征（PRRS）是由猪繁殖与呼吸综合征病毒（PRRSV）引起的一种严重传染病。PRRSV在全球范围内的流行对养猪业的发展产生了重大影响，造成了相当大的不利影响。PRRSV感染通常会导致宿主体内促炎因子大幅增加，如白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α，从而引发“炎症因子风暴”，导致猪肺部病变和严重病毒血症。目前，这种疾病尚无有效的治疗方法。最近的研究表明，中药具有显著的抗病毒作用。在本研究中，我们发现板蓝根多糖（IRPS）在体外能显著抑制PRRSV增殖。为探究这一现象的潜在原因，使用经修饰的猪肺泡巨噬细胞3D4/21/CD163细胞作为靶细胞，研究IRPS对PRRSV-BJ4的抑制作用。本研究表明，IRPS对PRRSV具有直接杀病毒活性，并能显著抑制PRRSV-BJ4毒株的入侵。采用间接免疫荧光法（IFA）、酶联免疫吸附测定法（ELISA）或蛋白质印迹法（Western blot）评估并确认PRRSV增殖、细胞因子分泌或Toll样受体4（TLR4）转录本及蛋白水平。研究发现，IRPS通过影响TLR4及其下游炎症信号通路来影响细胞因子分泌，从而防止炎症因子风暴的发生及随后PRRSV的感染。在本实验中，我们观察到IRPS在体外对PRRSV增殖具有显著抑制作用。这一发现为进一步研究中药的抗病毒特性奠定了理论基础。
