Ishii Kazuyuki, Yarita Hikaru, Manabe Shino
GlyTech, Inc., KRP#1-2F, 134 Chudoji Minamimachi, Shimogyo-ku, Kyoto 600-8813, Japan.
Laboratory of Synthetic Biomolecular Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Chem Pharm Bull (Tokyo). 2025;73(5):488-490. doi: 10.1248/cpb.c25-00091.
N-Glycosylated RNA (glycoRNA) has been identified on the cell surface, and 3-(3-amino-3-carboxypropyl)uridine has been reported as a conjugation site of N-glycans on RNA. Although the association of glycoRNAs with various diseases has been reported, their biosynthetic mechanisms and biological roles remain unexplored. Here, we report the preparation of two species of N-glycan-conjugated 3-(3-amino-3-carboxypropyl)uridine as the minimal units of glycoRNA. Our synthesized glycoRNA unit would contribute to future biochemical research on glycoRNAs.
N-糖基化RNA（糖基化RNA）已在细胞表面被鉴定出来，并且3-（3-氨基-3-羧丙基）尿苷已被报道为RNA上N-聚糖的结合位点。尽管已有报道称糖基化RNA与多种疾病有关，但其生物合成机制和生物学作用仍未被探索。在此，我们报告了两种N-聚糖共轭的3-（3-氨基-3-羧丙基）尿苷的制备，它们是糖基化RNA的最小单位。我们合成的糖基化RNA单位将有助于未来对糖基化RNA的生化研究。
