用于直接高效筛选适合生物胶结的细菌的平板上原位矿物形成
In situ mineral formation on a plate for direct and efficient screening of bacteria suitable for biocementation.
作者信息
Nakashima Kazunori, Sugawara Yuta, Fujita Masahiro, Yaegashi Aya, Chen Meiqi, Gowthaman Sivakumar, Nawarathna Thiloththama H K, Mwandira Wilson, Kamini Numbi Ramudu, Sujiritha Parthasarathy Baskaran, Takano Chikara, Kawasaki Satoru
机构信息
Division of Sustainable Resources Engineering, Faculty of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-Ku, Sapporo, 060-8628, Japan.
Department of Engineering Technology, University of Jaffna, Kilinochchi, 44000, Sri Lanka.
出版信息
Sci Rep. 2025 May 28;15(1):18706. doi: 10.1038/s41598-025-01350-2.
Microbially-induced carbonate precipitation (MICP) using a urea-hydrolyzing enzyme (urease) is a next-generation, environmentally friendly cementation technique. The isolation of excellent urease-producing bacteria for MICP is essential for establishing this technique. We have demonstrated a highly effective screening method for identifying promising bacteria suitable for MICP. In our selective plate, MICP-positive bacteria formed a CaCO halo around the colony on the plate, which was clearly distinguishable. Furthermore, we found that the CaCO formation activity of each bacterium was strongly correlated with the strength of the solidified sand samples. This technique is a novel approach for screening bacteria suitable for biocementation or MICP.
利用尿素水解酶（脲酶）进行微生物诱导碳酸盐沉淀（MICP）是一种新一代的环保固沙技术。分离出用于MICP的优良产脲酶细菌对于建立该技术至关重要。我们已经证明了一种高效的筛选方法，用于鉴定适合MICP的有前景的细菌。在我们的选择性平板上，MICP阳性细菌在平板上的菌落周围形成碳酸钙晕圈，这是明显可辨别的。此外，我们发现每种细菌的碳酸钙形成活性与固化砂样的强度密切相关。该技术是筛选适合生物胶结或MICP细菌的一种新方法。
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