Levy R, Warnke R, Dorfman R F, Haimovich J
J Exp Med. 1977 Apr 1;145(4):1014-28. doi: 10.1084/jem.145.4.1014.
Human tissues involved with lymphoma have been examined in frozen sections for immunoglobulin-bearing cells by a technique involving double-label immunofluorescence with mixed anti-kappa and anti-lambda antibodies. F (ab')2 fragments of purified antibodies were employed to avoid any binding via Fc receptors. B cell lymphomas were shown to be composed of monoclonal populations of Ig bearing cells, whereas normal or reactive lymphoid follicles contained a mosaic of Ig-bearing cells derived from multiple clones. Nodules of lymphoma were often surrounded by normal polyclonal B cell populations. We anticipates that the approach described here will be useful in the diagnosis of lymphoma, differentiating it from reactive lymphoid hyperplasia by the demostration of monoclonality. In addition, it should provide a sensitive and reliable tool for investigating the immunobiology of human lymphoma.
通过一种使用混合抗κ和抗λ抗体进行双标记免疫荧光的技术，对涉及淋巴瘤的人体组织冰冻切片进行了免疫球蛋白携带细胞检测。使用纯化抗体的F（ab'）2片段以避免通过Fc受体的任何结合。B细胞淋巴瘤显示为由携带Ig的细胞单克隆群体组成，而正常或反应性淋巴滤泡包含来自多个克隆的携带Ig细胞的镶嵌体。淋巴瘤结节常被正常多克隆B细胞群体包围。我们预期这里描述的方法将有助于淋巴瘤的诊断，通过证明单克隆性将其与反应性淋巴增生区分开来。此外，它应该为研究人类淋巴瘤的免疫生物学提供一个敏感且可靠的工具。
