Denariyakoon Sikrit, Giannoudis Athina, Puttipanyalears Charoenchai, Chatamra Kris, Mutirangura Apiwat
Queen Sirikit Centre for Breast Cancer, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok, 10330, Thailand.
Institute of Life Course and Medical Sciences, School of Dentistry, University of Liverpool, Sherrington Building, Ashton Street, Liverpool, L69 3GE, UK.
Asian Pac J Cancer Prev. 2025 May 1;26(5):1633-1640. doi: 10.31557/APJCP.2025.26.5.1633.
This study aimed to investigate the presence of cancer-associated senescent T cells in breast cancer patients and the impact of the progression of age on their senescent phenotypes.
The exhausted T cell lineage was excluded from the analysis of T cells by using PD1 marker. The percentages of CD28- cells were used to determine the senescent phenotype, in which the CD57 expression was used to further characterize their phenotypes. The flow cytometry was used on CytoFLEX flow cytometer and analysed with CytExpert analysis software.
In this study, both senescent and non-exhausted senescent T cells were significantly increased in breast cancer patients. However, non-exhausted T cells could demonstrate more significant proportion of senescent T cells, and these phenotypes in CD8+ T cells were increased in breast cancer patients, which were 53.03% and 37.25% (p<0.001). Moreover, CD28-CD57- cells of non-exhausted CD8+ T cells were increased irrespective of age, while the increase in CD28-CD57+ cells was positively correlated with the progression of age (r=0.353, p=0.015). In addition, the predominant CD28-CD57- cells were attenuated after 52 years of age.
The presence of non-exhausted senescent T cells seemed to be a concern regarding immune dysfunction in breast cancer patients. These phenotypes were presented in both young-age and old-age breast cancer, but the terminal phenotype seemed to be abundant in the elderly.
本研究旨在调查乳腺癌患者中癌症相关衰老T细胞的存在情况以及年龄进展对其衰老表型的影响。
通过使用PD1标志物将耗竭的T细胞谱系排除在T细胞分析之外。用CD28-细胞的百分比来确定衰老表型，其中用CD57表达进一步表征其表型。在CytoFLEX流式细胞仪上进行流式细胞术分析，并用CytExpert分析软件进行分析。
在本研究中，乳腺癌患者中衰老和未耗竭的衰老T细胞均显著增加。然而，未耗竭的T细胞中衰老T细胞的比例更高，且这些表型在乳腺癌患者的CD8+T细胞中增加，分别为53.03%和37.25%（p<0.001）。此外，未耗竭的CD8+T细胞中CD28-CD57-细胞的增加与年龄无关，而CD28-CD57+细胞的增加与年龄进展呈正相关（r=0.353，p=0.015）。此外，52岁以后，主要的CD28-CD57-细胞减少。
未耗竭的衰老T细胞的存在似乎是乳腺癌患者免疫功能障碍的一个问题。这些表型在年轻和老年乳腺癌患者中均有出现，但终末表型在老年人中似乎更为丰富。
