Thakare Eesha, Chaudhary Minal, Gawande Madhuri, Gadbail Amol Ramchandra, Zade Prajakta
Department of Oral Pathology and Microbiology, Nanded Rural Dental College, Nanded, Maharashtra, India.
Datta Meghe Institute of Medical Sciences (Deemed to be university), Nagpur (MS), India.
Asian Pac J Cancer Prev. 2025 May 1;26(5):1735-1743. doi: 10.31557/APJCP.2025.26.5.1735.
Evaluation and correlation of 17β-Estradiol(E2) in blood and Estrogen Receptor α (ERα) and Estrogen Receptor β(ER β) in tissue of four groups divided as normal control subjects (group I), active control subjects (group II), premalignant(leukoplakia) (group III) and OSCC patients (Group IV).
Subjects were divided in four groups mentioned previously and evaluated individually for 17β-estradiol(E2) in blood and ERα & ER β in tissue. The blood samples were evaluated by chemiluminescence assay and tissues samples were evaluated by Real-Time PCR for tissue analysis. Analysis of variance (ANOVA) was applied for quantitative evaluation of variables in each group followed by TukeyB test. Pearson Correlation Coefficient was used to study the correlation between the variables. Survival analysis was calculated by Kaplan-Meier Survival Analysis.
The results are indicative of statistically significant increased levels of E2 in the 4 groups by analysis of variance (p value-0.0327) followed by TukeyB test with statistically significant difference between Group VI and I (sig value-0.008) and Group VI and II (sig value-0.029). The Pearson correlation coefficient demonstrated that the mean levels of Estradiol are positively correlated with mean levels of ER α (p value- 0.030). Results did not indicate an association between survival and expression of E2, ER α and ER β.
The levels of E2 can be used as a marker in predicting the progression of disease from normal tissue not exposed to tobacco to normal tissue exposed to tobacco to premalignant to OSCC. The ERα in tissue is positively correlated with the increased levels of E2 in serum, so ERα expression in tissue along with E2 in serum could be used to identify the subsets of patients with higher risk of developing OSCC, especially those subjects with established tobacco habit but no appreciable change in oral mucosa.
评估并关联正常对照组（I组）、活性对照组（II组）、癌前病变（白斑）组（III组）和口腔鳞状细胞癌患者组（IV组）这四组人群血液中的17β-雌二醇（E2）以及组织中的雌激素受体α（ERα）和雌激素受体β（ERβ）。
将受试者分为上述四组，分别对其血液中的17β-雌二醇（E2）以及组织中的ERα和ERβ进行评估。血液样本通过化学发光法进行评估，组织样本通过实时荧光定量PCR进行组织分析。采用方差分析（ANOVA）对每组变量进行定量评估，随后进行TukeyB检验。使用Pearson相关系数研究变量之间的相关性。通过Kaplan-Meier生存分析计算生存率。
方差分析结果表明，四组中E2水平在统计学上显著升高（p值 - 0.0327），随后的TukeyB检验显示，VI组与I组（sig值 - 0.008）以及VI组与II组（sig值 - 0.029）之间存在统计学显著差异。Pearson相关系数表明，雌二醇的平均水平与ERα的平均水平呈正相关（p值 - 0.030）。结果未表明E2、ERα和ERβ的表达与生存率之间存在关联。
E2水平可作为预测疾病从未接触烟草的正常组织发展到接触烟草的正常组织、癌前病变再到口腔鳞状细胞癌进展的标志物。组织中的ERα与血清中E2水平的升高呈正相关，因此组织中的ERα表达以及血清中的E2可用于识别患口腔鳞状细胞癌风险较高的患者亚组，尤其是那些有既定烟草习惯但口腔黏膜无明显变化的受试者。
