Genomic analysis of the novel Stenotrophomonas phage vB_Srh_LBjhp91a, isolated from a Karst cave in China.

Stenotrophomonas phage vB_Srh_LBjhp91a was isolated from a karst cave, and its genome was sequenced. This phage has a 63,966-bp circular dsDNA genome (60.7% G + C content) containing 83 predicted open reading frames and two tRNAs. A BLASTn search revealed 80.95% nucleotide sequence identity to Stenotrophomonas phage vB_Sm_QDWS359 (genus Xooduovirus, family Mesyanzhinovviridae). Phylogenetic analysis based on the DNA polymerase and terminase large subunit genes grouped vB_Srh_LBjhp91a with members of the genera Bosavirus, Elanorvirus, and Xooduovirus. Proteomic phylogenetic analysis further clustered it with phage vB_Sm_QDWS359. Sequence comparisons revealed 71.7% average nucleotide identity between phage vB_Srh_LBjhp91a and phage vB_Sm_QDWS359. These findings indicate that phage vB_Srh_LBjhp91a should be considered a member of a new species within the genus Xooduovirus.