Ren Lou, Fang Zheng, Li Shixia, Wu Qingshan, Xiang Lan, Lin Chuangen, Liu Qiuping, Tan Leitao, Weng Qingbei
School of Life Sciences, Guizhou Normal University, Guiyang, 550025, PR China.
Qiannan Normal University for Nationalities, Duyun, 558000, PR China.
Arch Virol. 2025 May 29;170(7):142. doi: 10.1007/s00705-025-06329-y.
Stenotrophomonas phage vB_Srh_LBjhp91a was isolated from a karst cave, and its genome was sequenced. This phage has a 63,966-bp circular dsDNA genome (60.7% G + C content) containing 83 predicted open reading frames and two tRNAs. A BLASTn search revealed 80.95% nucleotide sequence identity to Stenotrophomonas phage vB_Sm_QDWS359 (genus Xooduovirus, family Mesyanzhinovviridae). Phylogenetic analysis based on the DNA polymerase and terminase large subunit genes grouped vB_Srh_LBjhp91a with members of the genera Bosavirus, Elanorvirus, and Xooduovirus. Proteomic phylogenetic analysis further clustered it with phage vB_Sm_QDWS359. Sequence comparisons revealed 71.7% average nucleotide identity between phage vB_Srh_LBjhp91a and phage vB_Sm_QDWS359. These findings indicate that phage vB_Srh_LBjhp91a should be considered a member of a new species within the genus Xooduovirus.
嗜麦芽窄食单胞菌噬菌体vB_Srh_LBjhp91a从一个喀斯特洞穴中分离得到，并对其基因组进行了测序。该噬菌体具有一个63,966碱基对的环状双链DNA基因组（G + C含量为60.7%），包含83个预测的开放阅读框和两个tRNA。BLASTn搜索显示，其与嗜麦芽窄食单胞菌噬菌体vB_Sm_QDWS359（Xooduovirus属，Mesyanzhinovviridae科）的核苷酸序列同一性为80.95%。基于DNA聚合酶和末端酶大亚基基因的系统发育分析将vB_Srh_LBjhp91a与Bosavirus属、Elanorvirus属和Xooduovirus属的成员归为一类。蛋白质组系统发育分析进一步将其与噬菌体vB_Sm_QDWS359聚类。序列比较显示，噬菌体vB_Srh_LBjhp91a与噬菌体vB_Sm_QDWS359之间的平均核苷酸同一性为71.7%。这些发现表明，噬菌体vB_Srh_LBjhp91a应被视为Xooduovirus属内一个新物种的成员。
