Cook Bradley W M, Hynes Alexander P
Cytophage Technologies Inc., Oak Bluff, MB, Canada.
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada.
NPJ Antimicrob Resist. 2025 May 29;3(1):45. doi: 10.1038/s44259-025-00117-z.
One of the biggest challenges in phage therapy is selecting the 'right' phage. What constitutes a 'good' phage is unclear, but regulators are converging on exclusion criteria for 'bad' phages. Here, we examine three commonly applied criteria: the lack of virulence/antibiotic resistance, the inability to transduce, and being strictly lytic (virulent). We assess the risk posed, the tools for determining the criteria, and the potential impact of the criteria.
噬菌体疗法面临的最大挑战之一是选择“合适的”噬菌体。什么样的噬菌体才是“好”噬菌体尚不清楚，但监管机构正在就“坏”噬菌体的排除标准达成共识。在此，我们研究三个常用标准：无毒性/抗生素抗性、无转导能力以及严格裂解（烈性）。我们评估这些标准所带来的风险、判定标准的工具以及这些标准的潜在影响。
