重新评估导致噬菌体不适合用于治疗的因素。
Re-evaluating what makes a phage unsuitable for therapy.
作者信息
Cook Bradley W M, Hynes Alexander P
机构信息
Cytophage Technologies Inc., Oak Bluff, MB, Canada.
Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada.
出版信息
NPJ Antimicrob Resist. 2025 May 29;3(1):45. doi: 10.1038/s44259-025-00117-z.
Abstract
One of the biggest challenges in phage therapy is selecting the 'right' phage. What constitutes a 'good' phage is unclear, but regulators are converging on exclusion criteria for 'bad' phages. Here, we examine three commonly applied criteria: the lack of virulence/antibiotic resistance, the inability to transduce, and being strictly lytic (virulent). We assess the risk posed, the tools for determining the criteria, and the potential impact of the criteria.
摘要
噬菌体疗法面临的最大挑战之一是选择“合适的”噬菌体。什么样的噬菌体才是“好”噬菌体尚不清楚，但监管机构正在就“坏”噬菌体的排除标准达成共识。在此，我们研究三个常用标准：无毒性/抗生素抗性、无转导能力以及严格裂解（烈性）。我们评估这些标准所带来的风险、判定标准的工具以及这些标准的潜在影响。
相似文献
1
Re-evaluating what makes a phage unsuitable for therapy.重新评估导致噬菌体不适合用于治疗的因素。
NPJ Antimicrob Resist. 2025 May 29;3(1):45. doi: 10.1038/s44259-025-00117-z.
2
Preclinical characterization and in silico safety assessment of three virulent bacteriophages targeting carbapenem-resistant uropathogenic Escherichia coli.三种靶向耐碳青霉烯类尿路致病性大肠杆菌的烈性噬菌体的临床前特性鉴定及计算机模拟安全性评估
Int Microbiol. 2024 Dec;27(6):1747-1763. doi: 10.1007/s10123-024-00508-8. Epub 2024 Mar 22.
3
The newly isolated lytic bacteriophages st104a and st104b are highly virulent against Salmonella enterica.新分离出的裂解性噬菌体st104a和st104b对肠炎沙门氏菌具有高度毒性。
J Appl Microbiol. 2006 Jul;101(1):251-9. doi: 10.1111/j.1365-2672.2005.02792.x.
4
Odorant-specific patterns of sniffing during imagery distinguish 'bad' and 'good' olfactory imagers.在想象过程中，特定气味的嗅闻模式可区分“不良”和“良好”的嗅觉想象者。
Chem Senses. 2005 Jul;30(6):521-9. doi: 10.1093/chemse/bji045. Epub 2005 Jul 19.
5
Transcriptomics-Driven Characterization of LUZ100, a T7-like Pseudomonas Phage with Temperate Features.基于转录组学的 LUZ100 特征分析，一种具有温和噬菌体特征的 T7 样假单胞菌噬菌体。
mSystems. 2023 Apr 27;8(2):e0118922. doi: 10.1128/msystems.01189-22. Epub 2023 Feb 16.
6
Two virulent Vibrio campbellii phages with potential for phage therapy in aquaculture.两种对水产养殖噬菌体疗法具有潜力的强毒坎氏弧菌噬菌体。
BMC Microbiol. 2025 Feb 28;25(1):99. doi: 10.1186/s12866-025-03803-0.
7
Phage Therapy: A Different Approach to Fight Bacterial Infections.噬菌体疗法：对抗细菌感染的一种不同方法。
Biologics. 2022 Oct 6;16:173-186. doi: 10.2147/BTT.S381237. eCollection 2022.
8
Genome Engineering of Virulent Lactococcal Phages Using CRISPR-Cas9.利用CRISPR-Cas9对烈性乳酸乳球菌噬菌体进行基因组工程改造
ACS Synth Biol. 2017 Jul 21;6(7):1351-1358. doi: 10.1021/acssynbio.6b00388. Epub 2017 Mar 30.
9
Resistance against two lytic phage variants attenuates virulence and antibiotic resistance in .对两种裂解噬菌体变体的抗性降低了 的毒力和抗生素耐药性。
Front Cell Infect Microbiol. 2024 Jan 17;13:1280265. doi: 10.3389/fcimb.2023.1280265. eCollection 2023.
10
Defining early seizure outcomes in pediatric epilepsy: the good, the bad and the in-between.定义小儿癫痫的早期发作结局：好的、坏的及介于两者之间的情况。
Epilepsy Res. 2001 Jan;43(1):75-84. doi: 10.1016/s0920-1211(00)00184-4.
引用本文的文献
1
Phage Therapy: Combating Evolution of Bacterial Resistance to Phages.噬菌体疗法：对抗细菌对噬菌体耐药性的演变
Viruses. 2025 Aug 8;17(8):1094. doi: 10.3390/v17081094.
2
Temperate phages increase antibiotic effectiveness in a infection model.温和噬菌体在感染模型中可提高抗生素的有效性。
mBio. 2025 Aug 18:e0162125. doi: 10.1128/mbio.01621-25.
3
Recent insights on challenges encountered with phage therapy against gastrointestinal-associated infections.噬菌体疗法治疗胃肠道相关感染所面临挑战的最新见解。
Gut Pathog. 2025 Aug 11;17(1):60. doi: 10.1186/s13099-025-00735-y.
4
Phage-Antibiotic Combinations for Pseudomonas: Successes in the Clinic and In Vitro Tenuously Connected.用于治疗假单胞菌的噬菌体-抗生素组合：临床与体外研究成果的微弱关联
Microb Biotechnol. 2025 Jul;18(7):e70193. doi: 10.1111/1751-7915.70193.
本文引用的文献
1
Prophages are infrequently associated with antibiotic resistance in clinical isolates.原噬菌体在临床分离株中很少与抗生素耐药性相关。
mSphere. 2025 Mar 25;10(3):e0090424. doi: 10.1128/msphere.00904-24. Epub 2025 Feb 13.
2
Temperate phage-antibiotic synergy is widespread-extending to -but varies by phage, host strain, and antibiotic pairing.温和噬菌体与抗生素的协同作用广泛存在，适用于多种情况，但会因噬菌体、宿主菌株和抗生素组合的不同而有所差异。
mBio. 2025 Feb 5;16(2):e0255924. doi: 10.1128/mbio.02559-24. Epub 2024 Dec 20.
3
VFDB 2025: an integrated resource for exploring anti-virulence compounds.VFDB 2025：一个用于探索抗毒力化合物的综合资源库。
Nucleic Acids Res. 2025 Jan 6;53(D1):D871-D877. doi: 10.1093/nar/gkae968.
4
Personalized bacteriophage therapy outcomes for 100 consecutive cases: a multicentre, multinational, retrospective observational study.100 例连续病例的个体化噬菌体治疗结果：一项多中心、多国、回顾性观察研究。
Nat Microbiol. 2024 Jun;9(6):1434-1453. doi: 10.1038/s41564-024-01705-x. Epub 2024 Jun 4.
5
Temperate phage-antibiotic synergy across antibiotic classes reveals new mechanism for preventing lysogeny.在不同抗生素类别中观察到温和噬菌体-抗生素协同作用，揭示了预防溶原性的新机制。
mBio. 2024 Jun 12;15(6):e0050424. doi: 10.1128/mbio.00504-24. Epub 2024 May 17.
6
Bacteriophages: Vectors of or weapons against the transmission of antibiotic resistance genes in hospital wastewater systems?噬菌体：医院废水处理系统中抗生素耐药基因传播的载体还是武器？
Water Res. 2024 Jan 1;248:120833. doi: 10.1016/j.watres.2023.120833. Epub 2023 Nov 7.
7
Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages rarely carry antibiotic resistance genes.对猪粪便样本中的病毒组和微生物组进行分析后发现，噬菌体和原噬菌体很少携带抗生素抗性基因。
ISME Commun. 2021 Oct 25;1(1):55. doi: 10.1038/s43705-021-00054-8.
8
Magistral Phage Preparations: Is This the Model for Everyone?顺势疗法制剂：这是每个人的理想模式吗？
Clin Infect Dis. 2023 Nov 2;77(Suppl 5):S360-S369. doi: 10.1093/cid/ciad481.
9
Translating phage therapy into the clinic: Recent accomplishments but continuing challenges.将噬菌体疗法转化为临床应用：近期的成就与持续的挑战。
PLoS Biol. 2023 May 23;21(5):e3002119. doi: 10.1371/journal.pbio.3002119. eCollection 2023 May.
10
Synergistic Interactions among Complex-Targeting Phages Reveal a Novel Therapeutic Role for Lysogenization-Capable Phages.复杂靶向噬菌体的协同相互作用揭示了溶原性噬菌体的一种新的治疗作用。
Microbiol Spectr. 2023 Jun 15;11(3):e0443022. doi: 10.1128/spectrum.04430-22. Epub 2023 May 17.
Zotero 插件