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对海洋阿斯加德古菌中含天线视紫红质的光捕获的结构见解。

Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea.

作者信息

Tzlil Gali, Marín María Del Carmen, Matsuzaki Yuma, Nag Probal, Itakura Shota, Mizuno Yosuke, Murakoshi Shunya, Tanaka Tatsuki, Larom Shirley, Konno Masae, Abe-Yoshizumi Rei, Molina-Márquez Ana, Bárcenas-Pérez Daniela, Cheel José, Koblížek Michal, León Rosa, Katayama Kota, Kandori Hideki, Schapiro Igor, Shihoya Wataru, Nureki Osamu, Inoue Keiichi, Rozenberg Andrey, Chazan Ariel, Béjà Oded

机构信息

Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel.

Physical and Analytical Chemistry Department, University of Jaén, Jaén, Spain.

出版信息

Nat Microbiol. 2025 Jun;10(6):1484-1500. doi: 10.1038/s41564-025-02016-5. Epub 2025 May 29.

DOI:10.1038/s41564-025-02016-5
PMID:40442502
原文链接:
https://pmc.ncbi.nlm.nih.gov/articles/PMC12137139/
Abstract

Aquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we show light energy transfer from diverse hydroxylated carotenoids to heimdallarchaeial rhodopsins (HeimdallRs) from uncultured marine planktonic members of 'Candidatus Kariarchaeaceae' ('Candidatus Asgardarchaeota'). These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light-absorbing retinal chromophore within HeimdallRs, enabling the use of light that is otherwise unavailable to the rhodopsin. Furthermore, we show elevated proton pumping by the antennas in HeimdallRs under white-light illumination, which better simulates the light conditions encountered by these archaea in their natural habitats. Our results indicate that light-harvesting antennas in microbial rhodopsins exist in families beyond xanthorhodopsins and proteorhodopsins and are present in both marine bacteria and archaea.

摘要

水生细菌视紫红质质子泵利用光能进行光异养生长，已知其含有羟基化类胡萝卜素，可扩展所利用光的波长范围，但这些羟基化类胡萝卜素在海洋古菌中尚未得到表征。在此，我们将海洋发色团提取物与在海洋宏基因组中鉴定出的纯化古菌视紫红质相结合，展示了光能从多种羟基化类胡萝卜素转移到来自未培养的“候选卡里古菌科”（“候选阿斯加德古菌门”）海洋浮游成员的海姆达尔古菌视紫红质（HeimdallRs）。这些光捕获天线在蓝光范围内吸收光，并将能量转移到HeimdallRs内吸收绿光的视黄醛发色团，使视紫红质能够利用原本无法利用的光。此外，我们还表明，在白光照射下，HeimdallRs中的天线可提高质子泵浦效率，这更能模拟这些古菌在其自然栖息地所遇到的光照条件。我们的结果表明，微生物视紫红质中的光捕获天线不仅存在于黄视紫红质和视紫质之外的家族中，而且在海洋细菌和古菌中均有存在。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/8573d4ede084/41564_2025_2016_Fig7_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/ef0934ef861a/41564_2025_2016_Fig8_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/e9dac4a924f8/41564_2025_2016_Fig9_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/f5747686a14d/41564_2025_2016_Fig10_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/2402823ac358/41564_2025_2016_Fig11_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/654a34e25ea1/41564_2025_2016_Fig12_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/454c98a8eb44/41564_2025_2016_Fig13_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/71a2e59a1d6c/41564_2025_2016_Fig14_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/0557d347222a/41564_2025_2016_Fig15_ESM.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f05c/12137139/1cd732345305/41564_2025_2016_Fig16_ESM.jpg

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