Mommaas-Kienhuis A M, van der Schroeff J G, Wijsman M C, Daems W T, Vermeer B J
Histochemistry. 1985;83(1):29-35. doi: 10.1007/BF00495296.
The morphological aspects of the binding and internalization of low density lipoproteins (LDL) and acetylated low density lipoproteins (AcLDL) by cultured human monocyte-derived macrophages were investigated. For this purpose, LDL and AcLDL were conjugated to 20 nm colloidal gold particles. After incubation of the cells with the conjugated lipoproteins at 4 degrees C some LDL- or AcLDL-gold complexes were found to be attached to the cell surface, but without characteristic localization. However, after incubation of the cells at 8 degrees C with either LDL-gold or AcLDL-gold, lipoprotein-gold complexes were present in clusters on the plasma membrane, often in coated pits. Cells incubated at 37 degrees C for various time periods showed internalization of both LDL- and AcLDL-gold complexes via small coated and non-coated vesicles and processing of the complexes in smooth-walled endosomes. When the cells were pulse-chased with LDL- or AcLDL-gold for 30 min at 37 degrees C, the gold conjugates occurred in dense bodies, probably lysosomes. The results suggest that although native and modified LDL are reported to be metabolized differently by macrophages, the morphological aspects of the endocytosis of LDL and AcLDL by cultured human monocyte-derived macrophages are similar.
研究了培养的人单核细胞衍生巨噬细胞对低密度脂蛋白（LDL）和乙酰化低密度脂蛋白（AcLDL）的结合及内化的形态学方面。为此，将LDL和AcLDL与20nm胶体金颗粒偶联。在4℃下将细胞与偶联的脂蛋白孵育后，发现一些LDL-或AcLDL-金复合物附着在细胞表面，但无特征性定位。然而，在8℃下将细胞与LDL-金或AcLDL-金孵育后，脂蛋白-金复合物以簇状存在于质膜上，常位于有被小窝中。在37℃下孵育不同时间段的细胞显示LDL-和AcLDL-金复合物通过小的有被和无被小泡内化，并在光滑壁的内体中处理复合物。当细胞在37℃下用LDL-或AcLDL-金脉冲追踪30分钟时，金偶联物出现在致密体中，可能是溶酶体。结果表明，尽管据报道天然和修饰的LDL被巨噬细胞代谢方式不同，但培养的人单核细胞衍生巨噬细胞对LDL和AcLDL的内吞作用的形态学方面是相似的。
