运动发酵单胞菌CP4中乙醇对发酵抑制的机制。
Mechanism of ethanol inhibition of fermentation in Zymomonas mobilis CP4.
作者信息
Osman Y A, Ingram L O
出版信息
J Bacteriol. 1985 Oct;164(1):173-80. doi: 10.1128/jb.164.1.173-180.1985.
Abstract
Accumulation of alcohol during fermentation is accompanied by a progressive decrease in the rate of sugar conversion to ethanol. In this study, we provided evidence that inhibition of fermentation by ethanol can be attributed to an indirect effect of ethanol on the enzymes of glycolysis involving the plasma membrane. Ethanol decreased the effectiveness of the plasma membrane as a semipermeable barrier, allowing leakage of essential cofactors and coenzymes. This leakage of cofactors and coenzymes, coupled with possible additional leakage of intermediary metabolites en route to ethanol formation, is sufficient to explain the inhibitory effects of ethanol on fermentation in Zymomonas mobilis.
摘要
发酵过程中酒精的积累伴随着糖转化为乙醇的速率逐渐降低。在本研究中，我们提供了证据表明乙醇对发酵的抑制作用可归因于乙醇对涉及质膜的糖酵解酶的间接影响。乙醇降低了质膜作为半透性屏障的有效性，导致必需的辅因子和辅酶泄漏。辅因子和辅酶的这种泄漏，再加上在乙醇形成过程中可能额外泄漏的中间代谢产物，足以解释乙醇对运动发酵单胞菌发酵的抑制作用。
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