Ito Kojiro, Tomita Shun, Fujimaki Takahiro, Matsutani Minenosuke, Enomoto Gen, Kajikawa Akinobu, Asai Kei, Yokota Kenji
Department of Agricultural Chemistry, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo, Hokkaido, 062-8571, Japan.
Arch Microbiol. 2025 May 30;207(7):163. doi: 10.1007/s00203-025-04357-5.
Bacillus velezensis TCG15 is a bacterial isolate from soil that shows antifungal activity against Aspergillus spp. and Candida spp. This study aimed to characterize its antifungal activity. TCG15 produces cyclic lipopeptides (cLPs), including surfactin A, plipastatin, and bacillomycin L. We obtained the complete sequence of the operon encoding nonribosomal peptide synthetase for bacillomycin L biosynthesis, bmyL operon (bmyLDABC), using genome analysis of TCG15. The bmyLD gene-disrupted mutant did not produce bacillomycin L, plipastatin, or surfactin A. The mutant showed no antifungal activity, indicating an essential role of cLPs. Among the cLPs, bacillomycin L was identified as the key antifungal compound. This activity was enhanced by surfactin A, which showed no antifungal activity alone. These findings suggested that the synergistic interaction between bacillomycin L and surfactin A contributed to the antifungal activity of TCG15.
贝莱斯芽孢杆菌TCG15是从土壤中分离出的一种细菌，对曲霉属和念珠菌属具有抗真菌活性。本研究旨在表征其抗真菌活性。TCG15产生环状脂肽（cLPs），包括表面活性素A、解脂素和杆菌霉素L。我们通过对TCG15的基因组分析，获得了编码杆菌霉素L生物合成的非核糖体肽合成酶操纵子的完整序列，即bmyL操纵子（bmyLDABC）。bmyLD基因破坏突变体不产生杆菌霉素L、解脂素或表面活性素A。该突变体没有抗真菌活性，表明cLPs起着至关重要的作用。在这些cLPs中，杆菌霉素L被确定为关键的抗真菌化合物。表面活性素A可增强这种活性，而表面活性素A单独不具有抗真菌活性。这些发现表明，杆菌霉素L和表面活性素A之间的协同相互作用有助于TCG15的抗真菌活性。
