脐带华通氏胶间充质基质/干细胞的分离
Isolation of Wharton's Jelly-Derived Mesenchymal Stromal/Stem Cells.
作者信息
Babbo Carina C, Durandt Chrisna, Ambele Melvin A, Pepper Michael S
机构信息
Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
出版信息
Methods Mol Biol. 2025;2938:21-29. doi: 10.1007/978-1-0716-4607-6_3.
Wharton's jelly-derived mesenchymal stromal/stem cells (WJSCs) are growing in popularity as an option for cell-based therapies. These cells are found within the gelatinous matrix of the umbilical cord, referred to as Wharton's jelly. WJSCs can be isolated by enzymatic tissue digestion or by using the explant method. Here, we discuss the explant method for the isolation of WJSCs.
源自华通氏胶的间充质基质/干细胞（WJSCs）作为基于细胞的治疗方法越来越受欢迎。这些细胞存在于脐带的凝胶状基质中，即华通氏胶。WJSCs可以通过酶促组织消化或采用组织块培养法分离。在此，我们讨论用于分离WJSCs的组织块培养法。
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