Adam Ladislaus Tapiwa, Pitere Reabetswe R, Ambele Melvin A, Pepper Michael S
Institute for Cellular and Molecular Medicine, Department of Immunology and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Methods Mol Biol. 2025;2938:63-67. doi: 10.1007/978-1-0716-4607-6_7.
Adipose-derived stromal/stem cells (ASCs) from human fat tissue samples offer a valuable model to study adipogenesis in vitro. A hormonal cocktail that promotes growth and differentiation can be used in experimental procedures to facilitate the conversion of ASCs into adipocytes. This protocol describes methods for adipogenic differentiation of primary human ASCs in vitro, outlining flow cytometry quantification and fluorescence microscopy qualitative assessment of the adipogenic process.
来自人类脂肪组织样本的脂肪来源基质/干细胞（ASCs）为体外研究脂肪生成提供了一个有价值的模型。一种促进生长和分化的激素混合物可用于实验程序，以促进ASCs向脂肪细胞的转化。本方案描述了原代人ASCs体外成脂分化的方法，概述了成脂过程的流式细胞术定量和荧光显微镜定性评估。
