脂肪生成过程中细胞内活性氧的测量

Measurement of Intracellular Reactive Oxygen Species During Adipogenesis.

作者信息

Adam Ladislaus Tapiwa, Ambele Melvin A, Pepper Michael S

机构信息

Institute for Cellular and Molecular Medicine, Department of Immunology and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

出版信息

Methods Mol Biol. 2025;2938:81-88. doi: 10.1007/978-1-0716-4607-6_9.

DOI:10.1007/978-1-0716-4607-6_9

PMID:40445282

Abstract

Reactive oxygen species (ROS) play an important role in the differentiation of immature stem cells to mature adipocytes in a process known as adipogenesis. Despite decades of study, there is still a gap in our understanding of the exact mechanisms played by ROS in regulating adipogenesis. This chapter describes flow cytometric measurement of endogenous ROS production during adipogenesis in adipose-derived stromal/stem cells (ASCs).

摘要

活性氧（ROS）在未成熟干细胞向成熟脂肪细胞分化的过程（即脂肪生成过程）中发挥着重要作用。尽管经过了数十年的研究，但我们对ROS在调节脂肪生成中所起的确切机制仍存在理解上的差距。本章描述了通过流式细胞术测量脂肪来源的基质/干细胞（ASC）脂肪生成过程中内源性ROS的产生情况。