Moela Pontsho
Division of Genetics, Faculty of Natural and Agricultural Sciences, Department of Biochemistry, Genetics, and Microbiology, University of Pretoria, Pretoria, South Africa.
Methods Mol Biol. 2025;2938:69-79. doi: 10.1007/978-1-0716-4607-6_8.
Western blotting (WB) is an end-point technique that is used to analyze the expression of target proteins in cells and tissues. It involves the separation of proteins using SDS-PAGE followed by an electrophoretic transfer of the proteins from the gel to a hydrophobic membrane where they will be immunodetected and captured on a film or by an imager. The peroxisome proliferator-activated receptor gamma (PPARγ) is a type of nuclear receptor protein that plays a crucial role in regulating gene expression and various cellular functions. In this chapter, the western blot procedure is demonstrated for the detection and quantification of the PPARγ nuclear protein from either adipocyte cell lines or adipose tissue.
蛋白质免疫印迹法（WB）是一种终点技术，用于分析细胞和组织中靶蛋白的表达。它包括使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离蛋白质，随后将蛋白质从凝胶电泳转移至疏水膜上，在该膜上它们将被免疫检测并捕获在胶片上或通过成像仪进行检测。过氧化物酶体增殖物激活受体γ（PPARγ）是一种核受体蛋白，在调节基因表达和各种细胞功能中起关键作用。在本章中，将演示蛋白质免疫印迹法检测和定量脂肪细胞系或脂肪组织中PPARγ核蛋白的操作步骤。
