Cheng Lijiao, Ding Junxiang, Liu Wenli, Li Yijie, Qin Chaobin, Yang Liping, Yan Xiao, Nie Guoxing
College of Fisheries, Henan Normal University, No. 46 Jianshe Road, Xinxiang, 453007, People's Republic of China.
College of Life Sciences, Henan Normal University, No. 46 Jianshe Road, Xinxiang, 453007, People's Republic of China.
Probiotics Antimicrob Proteins. 2025 May 30. doi: 10.1007/s12602-025-10589-0.
This research was conducted to evaluate the probiotic properties of Lactiplantibacillus plantarum (L. plantarum) P-8 through in vitro experiments and 12-week in vivo feeding trials, investigating its effects on growth performance, intestinal immunity, and microecology in Yellow River carp. In vitro experiments showed that L. plantarum P-8 inhibited various aquatic pathogenic bacteria and exhibited a certain level of safety in drug resistance tests. In the in vivo dosing feeding trial, fish (initial weight: 10.18 ± 0.01 g) were divided into five groups: CF, LP6, LP7, LP8, and Abx/LP8. Compared with CF group, the dietary addition of L. plantarum P-8 (1 × 10 CFU/g) in the LP8 and Abx/LP8 groups enhanced serum immune and antioxidant properties (P < 0.05), stimulated myofiber hyperplasia and hypertrophy through the promotion of muscle development (pax7, myf5, myod, myog, and mrf4) and suppression of muscle atrophy (mstnb) related gene expression (P < 0.05), and optimized the intestinal microbiota structure by increasing the abundance of Lactobacillus and Blautia, decreasing Flavobacterium and Helicobacter (P < 0.05), thereby improving intestinal health and growth performance. However, the LP6 and LP7 groups showed no significant health benefits. The pseudo-germ-free fish model (Abx/LP8 group) further demonstrated that L. plantarum P-8 can significantly modulate the structure and function of intestinal microbiota. Additionally, L. plantarum P-8's efficacy in reducing inflammation and fortifying the intestinal barrier was confirmed in faba bean-induced enteritis model. These evidences suggested that L. plantarum P-8 (1 × 10 CFU/g) improved intestinal health and growth performance by modulating the structure and function of intestinal microbiota.
本研究旨在通过体外实验和为期12周的体内喂养试验，评估植物乳杆菌（L. plantarum）P-8的益生菌特性，研究其对黄河鲤生长性能、肠道免疫和微生态的影响。体外实验表明，植物乳杆菌P-8对多种水产病原菌具有抑制作用，且在耐药性试验中表现出一定的安全性。在体内给药喂养试验中，鱼（初始体重：10.18±0.01 g）被分为五组：CF、LP6、LP7、LP8和Abx/LP8。与CF组相比，LP8和Abx/LP8组日粮中添加植物乳杆菌P-8（1×10 CFU/g）可增强血清免疫和抗氧化特性（P<0.05），通过促进肌肉发育（pax7、myf5、myod、myog和mrf4）和抑制肌肉萎缩（mstnb）相关基因表达刺激肌纤维增生和肥大（P<0.05），并通过增加乳酸杆菌和布劳特氏菌的丰度、降低黄杆菌和螺杆菌的丰度优化肠道微生物群结构（P<0.05），从而改善肠道健康和生长性能。然而，LP6和LP7组未显示出明显的健康益处。无菌鱼模型（Abx/LP8组）进一步证明植物乳杆菌P-8可显著调节肠道微生物群的结构和功能。此外，在蚕豆诱导的肠炎模型中证实了植物乳杆菌P-8在减轻炎症和强化肠道屏障方面的功效。这些证据表明，植物乳杆菌P-8（1×10 CFU/g）通过调节肠道微生物群的结构和功能改善了肠道健康和生长性能。
