Gao Pengfei, Hou Qiangchuan, Kwok Lai-Yu, Huo Dongxue, Feng Shuzhen, Zhang Heping
Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
Sci Bull (Beijing). 2017 Jan 30;62(2):105-113. doi: 10.1016/j.scib.2017.01.001. Epub 2017 Jan 4.
Poultry and eggs are nutritious and healthy foods that contain high-quality proteins and low levels of fat compared with other meats. Recent studies have shown that poultry performance is closely associated with colonic health. Antibiotics are commonly used in the poultry industry to control diseases and enhance survival rates. However, antibiotic use can also result in host gut dysbiosis and immune dysregulation. Such imbalances compromise poultry health and growth performance. Thus, our study investigated the effect of Lactobacillus (L.) plantarum P-8 on the gut microbiome of chickens co-inoculated with the antibiotic lincomycin. Principal coordinate analysis showed that L. plantarum P-8 treatment shifted the faecal bacterial population structure. At the metagenomic level, the Clusters of Orthologous Groups (COGs) functional categories of P, C, N and A were overrepresented in the probiotic group. Additionally, the relative gene abundances of metabolic pathways involved in flagellar assembly, bacterial chemotaxis, nitrogen metabolism, sulfur metabolism, cofactor and vitamin biosynthesis were also higher in the probiotic group than the control; in contrast genes related to galactose degradation, carbon fixation, multiple sugar transport systems and ribosomes were underrepresented in the probiotic group. Our data suggest that feeding L. plantarum P-8 has the potential to improve metabolic activity and nutrient utilization of poultry. Furthermore, the faecal antibiotic resistomes of the two groups could be separated using principal components analysis, indicating that the probiotic treatment may modulate the intestinal antibiotic resistance gene pool by changing the population structure of the gut microbiota. This study has provided interesting insights into the application of probiotics in the poultry industry.
家禽和蛋类是营养丰富且健康的食物，与其他肉类相比，它们含有优质蛋白质且脂肪含量低。最近的研究表明，家禽的生长性能与结肠健康密切相关。抗生素在禽类养殖业中常用于控制疾病和提高存活率。然而，抗生素的使用也会导致宿主肠道微生物群失调和免疫调节异常。这种失衡会损害家禽的健康和生长性能。因此，我们的研究调查了植物乳杆菌P-8对与抗生素林可霉素共同接种的鸡的肠道微生物群的影响。主坐标分析表明，植物乳杆菌P-8处理改变了粪便细菌种群结构。在宏基因组水平上，益生菌组中直系同源基因簇（COG）功能类别P、C、N和A的丰度过高。此外，益生菌组中参与鞭毛组装、细菌趋化性、氮代谢、硫代谢、辅因子和维生素生物合成的代谢途径的相对基因丰度也高于对照组；相比之下，与半乳糖降解、碳固定、多种糖转运系统和核糖体相关的基因在益生菌组中的丰度较低。我们的数据表明，饲喂植物乳杆菌P-8有可能提高家禽的代谢活性和营养利用率。此外，两组的粪便抗生素抗性组可以通过主成分分析分开，这表明益生菌处理可能通过改变肠道微生物群的种群结构来调节肠道抗生素抗性基因库。这项研究为益生菌在禽类养殖业中的应用提供了有趣的见解。
