聚乙二醇介导的脆弱拟杆菌与质粒DNA的转化
Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.
作者信息
Smith C J
出版信息
J Bacteriol. 1985 Oct;164(1):466-9. doi: 10.1128/jb.164.1.466-469.1985.
Abstract
A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for the preparation of frozen competent cells is also described.
摘要
通过使用克林霉素抗性质粒pBFTM10作为转化DNA的来源，开发了一种用质粒DNA转化脆弱拟杆菌的方法。该方法在技术上易于操作，每添加1微克pBFTM10平均产生4.2×10³个转化体。还描述了一种制备冷冻感受态细胞的方法。
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