枯草芽孢杆菌中染色体与质粒转化的比较研究：溶菌酶和聚乙二醇的作用
[Comparative study of chromosome and plasmid transformation in Bacillus subtilis: the effect of lysozyme and polyethylene glycol].
作者信息
Glumova E F, Prozorov A A
出版信息
Genetika. 1981;17(9):1581-7.
The influence of lysozyme and polyethyleneglycol (PEG) on chromosomal and plasmid transformation was comparatively investigated. Treatment of competent cell culture with low concentrations of lysozyme which do not affect cell viability stimulates both types of transformation. When transforming DNA was treated with definite concentrations of PEG, inhibition of transformation using chromosomal DNA occurred. The similar treatment, however, stimulated transformation by oligomeric forms of plasmid DNA. Possibly, PEG alters the DNA conformation rendering it less sensitive to nuclease degradation. This promotes plasmid transformation but reduces the frequency of transformation by chromosomal DNA, because the changes resulting from nuclease action further DNA integration into the genome of a recipient.
对溶菌酶和聚乙二醇（PEG）对染色体和质粒转化的影响进行了比较研究。用不影响细胞活力的低浓度溶菌酶处理感受态细胞培养物可刺激两种类型的转化。当用一定浓度的PEG处理转化DNA时，使用染色体DNA会抑制转化。然而，类似的处理会刺激质粒DNA寡聚体形式的转化。可能是PEG改变了DNA构象，使其对核酸酶降解的敏感性降低。这促进了质粒转化，但降低了染色体DNA转化的频率，因为核酸酶作用导致的变化进一步促进了DNA整合到受体基因组中。
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