枸杞叶的化学成分及其体外抗类风湿关节炎活性
Chemical constituents of Lycium barbarum leaves and their anti-rheumatoid arthritis activity in vitro.
作者信息
Wang Zi-Jiao, Tan Bang-Yin, Zhao Yun, Wang Chang-Bin, Zhao Yun-Li, Luo Xiao-Dong
机构信息
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, P. R. China.
University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
出版信息
Nat Prod Bioprospect. 2025 May 30;15(1):35. doi: 10.1007/s13659-025-00516-9.
Two new together with 32 known compounds were isolated from the leaves of Lycium barbarum. Their structures were elucidated using 1D and 2D NMR, HRESIMS, and ECD spectroscopic techniques. Compounds 1-34 were evaluated for their anti-rheumatoid arthritis activities in a lipopolysaccharide (LPS)-induced MH7A cells inflammatory model. As a result, compounds 1-3, 6, 8, 10, 14, 17-19, 29 and 31 inhibited the activity of lactate dehydrogenase (LDH) and nitric oxide (NO) at concentrations 20 μM. Among them, compound 1 showed the best effectiveness, with inhibition rates of 46.7% for NO and 32.8% for LDH.
从枸杞叶中分离出2个新化合物和32个已知化合物。采用一维和二维核磁共振、高分辨电喷雾电离质谱及电子圆二色谱等光谱技术对其结构进行了鉴定。在脂多糖（LPS）诱导的MH7A细胞炎症模型中，对化合物1-34的抗类风湿关节炎活性进行了评价。结果显示，化合物1-3、6、8、10、14、17-19、29和31在浓度为20μM时可抑制乳酸脱氢酶（LDH）和一氧化氮（NO）的活性。其中，化合物1的效果最佳，对NO的抑制率为46.7%，对LDH的抑制率为32.8%。
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