Huang Kang, Lu Shijuan, Zhou Yilei, Lin Dehong, Chen Zibin, Zhong Zanrui, Zhong Jianghua
Department of Cardiology, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, 43 People's Avenue, Haidian Island, Haikou, 570208, Hainan, China.
Cardiovasc Toxicol. 2025 Jul;25(7):1042-1054. doi: 10.1007/s12012-025-10003-9. Epub 2025 May 30.
Coronary heart disease (CHD) is a prevalent cardiovascular' condition characterized by high morbidity and mortality rates, with a significant genetic component. The VEGFA gene plays a crucial role in the development of CHD. This study aims to evaluate the influence of VEGFA gene polymorphisms on CHD susceptibility. Peripheral blood samples were collected from 479 CHD patients and 479 healthy controls. We genotyped four VEGFA single nucleotide polymorphisms (SNPs): rs833068, rs833070, rs3025021, and rs3025030, using the Agena MassARRAY platform. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to evaluate the association between these SNPs and CHD risk. Multifactor dimensionality reduction (MDR) was employed to analyze potential SNP-SNP interactions. The rs833068 SNP was found to be associated with a reduced risk of CHD (OR 0.46, p = 0.013), whereas the rs3025021 (OR 1.32, p = 0.031) and rs3025030 (OR 1.30, p = 0.032) SNPs were linked to an increased risk. The stratified analysis revealed that rs833068 decreased CHD risk in individuals aged < 61 years, those with a BMI ≥ 24, non-smokers, non-drinkers, and patients with diabetes, but increased the risk in hypertensive individuals. The rs833070 SNP significantly increased CHD risk in non-smokers and non-drinkers. Rs3025021 was associated with an increased CHD risk in subjects with a BMI < 24, smokers, drinkers, and diabetics. Rs3025030 was found to increase CHD susceptibility in aged > 61 years, males, those with a BMI ≥ 24, and drinkers. MDR analysis identified a combination of rs833068, rs3025021, and rs3025030 as the most predictive model for CHD. Polymorphisms in the VEGFA gene are associated with CHD risk in the Chinese Han population, offering a novel perspective for CHD diagnosis.
冠心病（CHD）是一种常见的心血管疾病，其发病率和死亡率很高，且具有显著的遗传成分。血管内皮生长因子A（VEGFA）基因在冠心病的发生发展中起关键作用。本研究旨在评估VEGFA基因多态性对冠心病易感性的影响。收集了479例冠心病患者和479例健康对照者的外周血样本。我们使用Agena MassARRAY平台对四个VEGFA单核苷酸多态性（SNP）进行基因分型：rs833068、rs833070、rs3025021和rs3025030。计算优势比（OR）和95%置信区间（CI）以评估这些SNP与冠心病风险之间的关联。采用多因素降维法（MDR）分析潜在的SNP-SNP相互作用。发现rs833068 SNP与冠心病风险降低相关（OR 0.46，p = 0.013），而rs3025021（OR 1.32，p = 0.031）和rs3025030（OR 1.30，p = 0.032）SNP与风险增加相关。分层分析显示，rs833068降低了年龄<61岁、体重指数（BMI）≥24、不吸烟者、不饮酒者和糖尿病患者的冠心病风险，但增加了高血压患者的风险。rs833070 SNP显著增加了不吸烟者和不饮酒者的冠心病风险。rs3025021与BMI<24、吸烟者、饮酒者和糖尿病患者的冠心病风险增加相关。发现rs3025030增加了年龄>61岁、男性、BMI≥24和饮酒者的冠心病易感性。MDR分析确定rs833068、rs3025021和rs3025030的组合是冠心病最具预测性的模型。VEGFA基因多态性与中国汉族人群的冠心病风险相关，为冠心病诊断提供了新的视角。
