Sharma Nishant, Sharma Nevadita, Biswas Ashis, Gupta Shamba, Behura Assirbad, Rodriguez Gloria Marcela
The Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, United States of America.
PLoS One. 2025 May 30;20(5):e0324919. doi: 10.1371/journal.pone.0324919. eCollection 2025.
Mycobacterium tuberculosis, the pathogen responsible for human tuberculosis, responds to iron limitation by increasing the production of extracellular vesicles. This study examined the protein composition of induced M. tuberculosis extracellular membrane vesicles using chromatography coupled with mass spectrometry. The results revealed that vesicles contain key pathogenicity factors, including proteins that enhance bacterial survival, immune evasion, and inflammation. These findings deepen our understanding of the potential role of extracellular vesicles in M. tuberculosis-host interactions. The data can also aid in identifying new biomarkers of infection and developing vesicle-based, culture-independent TB diagnostic platforms.
结核分枝杆菌是导致人类结核病的病原体，它通过增加细胞外囊泡的产生来应对铁限制。本研究使用色谱联用质谱法检测了诱导产生的结核分枝杆菌细胞外膜囊泡的蛋白质组成。结果显示，囊泡含有关键的致病因子，包括增强细菌存活、免疫逃避和炎症反应的蛋白质。这些发现加深了我们对细胞外囊泡在结核分枝杆菌与宿主相互作用中潜在作用的理解。这些数据还有助于识别新的感染生物标志物，并开发基于囊泡的、无需培养的结核病诊断平台。
