Mhanna Mariam A, Gauthier David T, Shollenberger Lisa M
Department of Biological Sciences, College of Sciences, Old Dominion University, Virginia, United States of America.
Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Georgia, United States of America.
PLoS One. 2025 May 30;20(5):e0324638. doi: 10.1371/journal.pone.0324638. eCollection 2025.
Eggs of Schistosoma mansoni are produced by adult female worms in mesenteries of infected hosts. Eggs can cross the intestinal barrier and form granulomas in the tissue or breach and exit the host through fecal excretion. These interactions may affect the host microbiome assemblages. Given the potential for schistosomal alteration of host gut microbiome and subsequent effects on the fecal bacterial composition, it is important to conduct controlled microbiome studies on model animals. While pursuing these studies, it is important to take into account the different conditions in which microbiome studies are conducted and their consequent impacts on variability and reproducibility of results. In particular, we are interested in inter-institutional effects on controlled microbiome studies, in which the study location itself may impact study outcomes. In this work, we report global changes caused by acute and chronic schistosomiasis on the fecal microbiome of mice at two different institutions and three timepoints.
曼氏血吸虫卵由受感染宿主肠系膜中的成年雌虫产生。虫卵可穿过肠道屏障，在组织中形成肉芽肿，或破溃并通过粪便排出宿主。这些相互作用可能会影响宿主微生物群落组合。鉴于血吸虫对宿主肠道微生物群的潜在改变以及随后对粪便细菌组成的影响，在模式动物上进行对照微生物组研究很重要。在进行这些研究时，重要的是要考虑进行微生物组研究的不同条件及其对结果变异性和可重复性的影响。特别是，我们对机构间对对照微生物组研究的影响感兴趣，其中研究地点本身可能会影响研究结果。在这项工作中，我们报告了急性和慢性血吸虫病在两个不同机构的三个时间点对小鼠粪便微生物组造成的全局变化。
