Vincent A, Stange K, Louveau I, Röntgen M, Dessauge F
PEGASE, INRAE, Institut Agro, 35590, Saint-Gilles, France.
Research Institute for Farm Animal Biology (FBN), Cell Biology of Muscle Growth, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.
Differentiation. 2025 Jul-Aug;144:100871. doi: 10.1016/j.diff.2025.100871. Epub 2025 May 27.
Skeletal muscle mesenchymal stromal cells (MSCs) are resident cells in the stromal, interstitial or perivascular areas, with satellite cells (SCs) acting as essential precursors for muscle growth and regeneration. This study firstly aimed to phenotype freshly isolated SCs using cell surface markers and gene expression and to assess their ability to differentiate in myogenic or adipogenic conditions. Then, refined SCs populations sorted according to the myogenic CD56 cell surface marker were characterized. SCs were isolated from the longissimus muscle of 5-7-day-old piglets and analyzed using flow cytometry. The hematopoietic CD45 + cells represented 20 % of the total isolated cell population. The myogenic CD29 and CD56 positive cell populations were the most abundant (80 % and 50 % respectively). Mesenchymal CD90 positive cells were also highly present (34 %) while the proportions of fibro-adipogenic CD140a and CD34-positive cells were low (<3 %). In this study, we showed that total isolated muscle-derived SCs were able to differentiate into myotubes in both myogenic and adipogenic media. Transcriptional profiles were similar, except for PPARγ, PGC1α, and Myosin Heavy Chain 2B witch present a higher induction during differentiation in the adipogenic medium. Interestingly, non-hematopoietic CD45sorted cells further separated in CD45-/CD56+ and also CD45-/CD56-sub-populations mostly formed myotubes under both conditions, with CD56cells showing potential and gene expression profile of myoblasts. Mature adipocytes were found in the CD45-/CD56+ group after differentiation in adipogenic medium. This study increases knowledge on myogenic cell surface marker and underscores the complexity and heterogeneity of muscle SCs.
骨骼肌间充质基质细胞（MSCs）是存在于基质、间质或血管周围区域的驻留细胞，卫星细胞（SCs）是肌肉生长和再生的重要前体细胞。本研究首先旨在利用细胞表面标志物和基因表达对新鲜分离的SCs进行表型分析，并评估它们在成肌或成脂条件下的分化能力。然后，对根据成肌CD56细胞表面标志物分选的纯化SCs群体进行了表征。从5 - 7日龄仔猪的背最长肌中分离SCs，并使用流式细胞术进行分析。造血CD45 +细胞占分离的总细胞群体的20%。成肌CD29和CD56阳性细胞群体最为丰富（分别为80%和50%）。间充质CD90阳性细胞也大量存在（34%），而成纤维脂肪生成CD140a和CD34阳性细胞的比例较低（<3%）。在本研究中，我们表明分离的总肌肉来源SCs能够在成肌和成脂培养基中分化为肌管。转录谱相似，除了PPARγ、PGC1α和肌球蛋白重链2B在成脂培养基分化过程中诱导更高。有趣的是，非造血CD45分选细胞进一步分为CD45 - /CD56 +和CD45 - /CD56 -亚群，在两种条件下大多形成肌管，CD56细胞显示出成肌细胞的潜能和基因表达谱。在成脂培养基中分化后，在CD45 - /CD56 +组中发现了成熟脂肪细胞。本研究增加了对成肌细胞表面标志物的认识，并强调了肌肉SCs的复杂性和异质性。
