Schatz R W, Soto A M, Sonnenschein C
J Cell Physiol. 1985 Sep;124(3):386-90. doi: 10.1002/jcp.1041240305.
We have previously reported that the proliferation of cloned MCF-7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal-dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 X 10(-11) M estradiol-17 beta to the culture media. These observations suggest that the proliferation of T47D and MCF-7 cells is regulated by a blood-borne inhibitor whose effects are neutralized by estrogens. In the present report we explore the possibility that progesterone alters the estrogenic response. MCF-7 cells were grown in DME containing 2-40% CDFHS. Progesterone, at 3 X 10(-7) M to 3 X 10(-12) M, had no effect on the yield of MCF-7 or T47D cells that were cultured in the presence or absence of estradiol-17 beta.
我们之前曾报道，通过增加经活性炭-葡聚糖处理的女性人血清（CDFHS）的浓度，可抑制克隆的MCF-7和T47D人乳腺肿瘤细胞的增殖。通过向培养基中添加3×10⁻¹¹ M的17β-雌二醇可恢复最大增殖率。这些观察结果表明，T47D和MCF-7细胞的增殖受一种血源性抑制剂的调节，其作用可被雌激素中和。在本报告中，我们探讨了孕酮改变雌激素反应的可能性。MCF-7细胞在含有2 - 40% CDFHS的DME中培养。孕酮浓度在3×10⁻⁷ M至3×10⁻¹² M之间，对在有或没有17β-雌二醇存在的情况下培养的MCF-7或T47D细胞的产量没有影响。
